Journal of Cellular Biotechnology - Volume 3, issue 2
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Journal of Cellular Biotechnology is a peer-reviewed international journal for advancing research activities in the field of cellular biotechnology. It serves as a medium for the publication of full papers, invited reviews, short communications, technical notes and letters to the Editor-in-Chief on all aspects of cellular biotechnology. This comprises molecular biological topics covering biochemical, chemical, pharmacological or bioprocess engineering aspects, as well as the development of novel biomaterials. Therefore, cellular biotechnology differs from biology, biochemistry, and other basic life sciences by its emphasis on using the knowledge of bioscience to solve important practical problems. Papers presenting information of a multidisciplinary nature - not suitable for publication in a journal devoted to a single discipline - are particularly welcome.
Manuscripts submitted for the
Journal of Cellular Biotechnology are expected to cover activities related to molecular diagnostics, the expansion of human primary cells for individualized therapies or drug testing, 2- and 3-dimensional co-culture techniques, cell line validation, tissue engineering, and stem cell biology for the treatment of human pathologies. This includes studies on the design of reactors and research on cellular biology and physiology of mammalian cells in vitro and in vivo, and tissue. Of special interest is the rational manipulation of reactions through metabolic engineering techniques or specific reactor operations that lead to biomaterials with unique properties. Also, biochemical and physiological studies of metabolism and enzymes as relevant for tissue culture cells, investigations at the molecular level including transcription/translation control; design and engineering of products by molecular strategies; engineering of cellular modification and transport systems such as post-translational protein modifications as well as protein and metabolite secretion; molecular strategies of screening for new or modified products (e.g. pharmaceuticals or bioactive compounds). In addition, investigations in preclinical animal experiments are welcome.
The endeavour of the Editor-in-Chief and publisher of the
Journal of Cellular Biotechnology is to bring together contributions from those working in various fields related to cell-cell or cell-material interactions all over the world. The editorial board members of the
Journal of Cellular Biotechnology are from those countries in Europe, Asia, Australia and America where appreciable work in cellular biotechnology is being carried out. Each editor takes responsibility to decide on the acceptance of a manuscript. He/she is required to have the manuscript appraised by two referees and may be one of them himself. The executive editorial office, to which the manuscripts have been submitted, is responsible for rapid handling of the reviewing process.
Abstract: Erythrocytes transport oxygen from the lungs to the tissues. The excess surface area together with the elasticity of the erythrocyte cell membrane provides the flexibility needed to pass through the microvasculature where the oxygen exchange occurs. Although the architecture of the red cell and its membrane-associated cytoskeletal network is known in general, the factors that control the characteristic shape change during echinocyte formation are poorly understood. In this short report we show that in echinocytes a completely reorganized membrane cytoskeleton with a box-like structure of actin filaments prevailed indicating the importance of the actin cytoskeleton during echinocyte formation.
Abstract: Drug induced liver injury (DILI) is still the leading single cause of drug failure during clinical phases and after market approval. Currently, many laboratories aim to develop appropriate in vitro systems to predict drug hepatotoxicity. Primary human hepatocytes are still the gold standard, but they have substantial disadvantages such as rapid dedifferentiation in vitro and lack of cell proliferation. In addition to primary human hepatocytes, liver cancer-derived cell lines such as HepG2, cytochrome P450 (CYP450) overexpressing HepG2 cell clones and HepaRG were studied intensively. In contrast to HepG2, HepaRG show promising characteristics of differentiated primary human hepatocytes, but…they represent only one donor. There is some hope that this lack of donor variability can be solved by the use of iPS-derived hepatocytes. However, iPS technology still seems to need some improvement to produce physiologically relevant hepatocytes. Upcyte hepatocytes represent the most recent technical advancement combining some features of primary human hepatocytes such as physiological activity and donor variability with the ability of cell lines for extended proliferation. Altogether, more work is needed to develop and validate appropriate in vitro systems for precise prediction of DILI risk.
Abstract: BACKGROUND: The cumulating long-term changes in stable macromolecules, including non-enzymatic glycosylation of proteins and oxidative stress, are main fundamental reasons for development of late complications in patients with diabetes mellitus type II. OBJECTIVE: To evaluate some high-sensitive and specific proteins as early diagnostic tool and prognostic biomarkers of late complications of diabetes mellitus type 2 (T2DM). METHODS: Proteomic studies of blood plasma in patients with T2DM were performed through one- and two-dimensional electrophoresis of proteins in polyacrylamide gel, in the presence of sodium dodecyl sulfate - according to Laemmli (SDS PAGE). RESULTS: The spectrum…of 70 proteins was proposed as tools in clinical/laboratory diagnostics and as independent prognostic biomarkers - predicting early development of disease complications. CONCLUSIONS: The identified proteomic biomarkers in blood plasma of patients with T2DM could serve as drug targets for early prevention and successful treatment of disease complications.
Keywords: Proteomic biomarkers in blood plasma, one- and two- dimensional electrophoresis, late complications of diabetes mellitus type II
Abstract: A recent study showed that polymerized siloxane (PDMS) coating of biomaterials like polycarbonate, stainless steel or glass results in a hydrophobic and positively charged surface, which is known to be advantageous for cell adhesion. However, when Escherichia coli and Staphylococcus epidermidis were cultured on PDMS coated materials for one hour, this resulted in a significant decrease of bacterial adherence compared to non-coated materials. The study therefore aimed at investigating antimicrobial effects of PDMS. We used nosocomial aerobic grown bacteria S. epidermidis, Pseudomonas aeruginosa, E. coli and the biofilm formation model organism Bacillus subtilis , which were exposed to…PDMS in a planktonic culture assay and additionally while biofilm formation. PDMS had a significant impact on the growth of these bacteria in both culture assays. In planktonic culture, PDMS exposition resulted in a decreased growth of all bacteria tested, which was strongly species specific. Biofilm culture in contrast caused an increased growth of E. coli and P. aeruginosa , while growth of S. epidermidis and B. subtilis was decreased. However, these results are based on tests of single species biofilms. Previous to practical application it is necessary to confirm these results by tests in which different bacterial species are able to interact like in natural biofilms resulting in modified bacterial growth behavior and toxin resistance.
Abstract: Nowadays the importance of the peripheral blood stem cell (PBSC) donation is continuously increasing. It is therefore crucial to determine all side effects for donors. This clinical trial deals with the preventative therapy using Allopurinol (300 mg/d) in order to prohibit the increase in serum uric acid as a result of rhG-CSF injections (10 μg/kg Lenograstim over 5d). It comprises 72 donors (m/f: 57/15) passing through 3 examinations. The initial measurement of uric acid serves as clue to categorize the donors into groups. Donors with high uric acid are summarized as group 1 (30: m/f: 27/3), which obtained Allopurinol, whereas donors…with normal uric acid are part of group 4 (42: m/f: 30/12). This group is additionally divided into groups 2 and 3 (dependent on the way of G-CSF administration). The mean serum uric acid of group 1 decreased from 7.01 to 5.03 mg/dl. In contrast, the value of group 4 increased from 5.16 to 6.15 mg/dl.