Clinical Hemorheology and Microcirculation - Volume 63, issue 4

Authors: Lima, Catarina | Pinto, Susana | Napoleão, Patrícia | Pronto-Laborinho, Ana Catarina | Barros, Maria Amparo | Freitas, Teresa | de Carvalho, Mamede | Saldanha, Carlota

Article Type: Research Article

Abstract: INTRODUCTION: Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease of the motor system. It has been hypothesised that red blood cells (RBCs) may be involved in the disease process by the release of damaging molecules. OBJECTIVE: The aim of this ex vivo study is to compare RBCs biochemical and hemorheological parameters between ALS patients and healthy donors to identify novel biomarkers of the ALS disease. METHODS: We included 82 ALS patients and 40 gender age-matched healthy donors. We performed quantification of erythrocyte aggregation and deformability, nitric oxide (NO) efflux from RBCs, acetylcholinesterase (AChE) …enzyme activity and intraerythrocytic concentration of nitrite, nitrate and S-nitrosogluthatione (GSNO). RESULTS: Erythrocyte deformability and AChE activity were increased in patients with ALS in comparison to healthy donors. NO efflux from RBCs and concentration of intraerythrocytic nitrite were lower in ALS patients. In patients, we found that for higher NO range of values the respiratory function is worse and that for higher AChE range of values the RBCs nitrite content increase. CONCLUSION: The results of the present study indicate that NO efflux from RBCs and RBCs AChE should be further explored as potential biomarkers for ALS. Show more

Keywords: Acetylcholinesterase, amyotrophic lateral sclerosis, erythrocyte, nitric oxide, nitrite, respiratory function

DOI: 10.3233/CH-162066

Citation: Clinical Hemorheology and Microcirculation, vol. 63, no. 4, pp. 423-437, 2016

Authors: Kuehlmann, Britta | Prantl, Lukas

Article Type: Research Article

Abstract: AIM: To identify a possible connection between anaplastic large cell lymphoma and different types of breast implants. METHODS: We conducted a retrospective evaluation of 296 breast tissues of 227 women with different breast implant types undergoing surgical revision or explantation between January 2000 and June 2015. Histological and selected immunohistochemical analyses of CD30-&ALK-1-markers of the breast capsules were performed. RESULTS: The womens’ average age was 42.91±12.66 years (median: 43.83 years) during implantation and 51.40±11.40 years (median: 52.37 years) during revision or explantation of the implants. Average implant residing time was 8.49±8.90 years (median: 5.83 years). …In 51% implantation was for reconstructive, in 48% for aesthetic reasons, in 1% for other reasons. At 59% the main reason for explantation or removal was capsular fibrosis (n  = 173). In 296 breast capsules we could not find pathological lymphoma cells according to ALCL, retrospectively. CONCLUSION: In our study we detected high incidences of various cells in relationship to the implant’s type and residing time, which will be published in further articles. We could not find ALCL-cells in breast capsules of explanted or revised breast implants during 2000–2015, retrospectively. There should be a heightened awareness of a possible relationship between the development of cancer and breast implants. To date there are case reports about a possible association between the development of ALCL and breast implants. The number of cases are few and our knowledge of the pathogenesis is little. Further investigation is needed to understand the possible link between breast implants and ALCL found in the breast. Show more

Keywords: Breast implants, ALCL, histological and immunohistochemical analyses, CD30

DOI: 10.3233/CH-162071

Citation: Clinical Hemorheology and Microcirculation, vol. 63, no. 4, pp. 439-449, 2016

Authors: Moon, Hwang-Woon | Shin, Se-Hyun | Lee, Chul-Hyun | Park, Hun-Young | Sunoo, Sub | Nam, Sang-Seok

Article Type: Research Article

Abstract: Even though exercise hemorheology at hypoxic condition has been considered as a good tool to understand clinical hemorheology, there have been limited studies reported. Previous researches showed that hemorheological variables are closely correlated with oxygen delivery capacity during exercise. The present study investigated hypoxic responses including RBC deformability and aggregation, metabolic parameters and complete blood cell counts at various hypoxic conditions during cycling exercise and recovery. Eleven Korean healthy male subjects performed submaximal bike exercise at sea level (20.9% O2 ) and under various hypoxic conditions (16.5% O2 , 14.5% O2 , 12.8% O2 , and 11.2% O2 ) in …a random order. The submaximal bike exercise intensity of the subjects was 70% maximum heart rate at sea level. All variables were measured at rest, during exercise and recovery 30-minute, respectively. As oxygen partial pressure decreased, arterial blood oxygen saturation decreased but oxygen uptake did not change much. Heart rate and lactate concentration during exercise increased when oxygen partial pressure is less than or equal to 14.5% O2 condition. Red blood cell (RBC) counts, hemoglobin counts, and hematocrit level were not apparently altered with hypoxic conditions. RBC deformability showed significant alterations at 11.2% O2 conditions compared with other hypoxic conditions during exercise or recovery, except at 10 minutes recovery. However, decreases in oxygen partial pressure did not affect red blood cell aggregation. Therefore, we conclude that alterations in RBC deformability may reduce aerobic capabilities at hypoxic condition. Show more

Keywords: Altitude, Hypoxic condition, RBC Deformability, RBC Aggregation, Metabolic parameter, Exercise

DOI: 10.3233/CH-16163

Citation: Clinical Hemorheology and Microcirculation, vol. 63, no. 4, pp. 451-460, 2016

Article Type: Other

Citation: Clinical Hemorheology and Microcirculation, vol. 63, no. 4, pp. 461-466, 2016