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Price: EUR 185.00Authors: Nash, Gerard B. | Meiselman, Herbert J.
Article Type: Research Article
Abstract: The mechanical characteristics of the structural components of polymorphonuclear granulocytes (PMN) and lymphocytes were tested by aspirating tongues from these cells into micropipettes with internal diameter (Dp) > ∼ 2um. PMN tongues deformed in a continuous manner, while lymphocyte deformation was slower and approached a limiting steady level. The greater rigidity of the lymphocytes appeared to arise from their large, solid nucleus, while the PMN cytoplasm acted as a viscous fluid at these large scale deformations. Overall WEC deformability was also tested by measuring the time required for complete cell entry (te) into …pipettes with Dp = 4 to 6um. The relative ease of entry of PMN and lymphocytes depended on the pipette diameter; lymphocytes entered more slowly than PMN for Dp = 4um, but more quickly than PMN for Dp = 5.9um. The predicted threshold pressure at which entry could just be induced was greater for lymphocytes than for PMN at Dp < 5um, but similar for the two cell types for Dp > ∼ 5um. Apparently the greater stiffness of lymphocytes makes it relatively difficult for them to enter the smaller pipettes, but for the larger pipettes their smaller volume allows them to enter more rapidly than the PMN. Thus, the relative contributions of PMN and lymphocytes to blood flow resistance are likely to depend strongly on the vessel size. It was also noted that active PMN with pseudopodia took much longer to enter pipettes than smooth, passive PMN, therefore indicating that rheological tests on white blood cells must consider the possibility of cell activation. Show more
Keywords: White Blood Cell Rheology, Flow Resistance
DOI: 10.3233/CH-1986-6110
Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 1, pp. 87-97, 1986
Authors: Palareti, G. | Finelli, C. | Poggi, M. | Tricarico, M.G. | Fiacchini, M. | Ricci, P. | Tura, S. | Coccheri, S.
Article Type: Research Article
Abstract: Haemorheologic studies, were performed in 68 patients (pts) with Polycythaemia Vera (PV) under treatment by venesection alone or by cytostatic drugs with or without venesection. PV pts had higher haematocrit (PVC) and elevated whole blood viscosity (WBV) even after reconstitution of blood to 0.45 PCV, in comparison with a control group. MCV and MCH were parallely reduced. In the pts with marked hypochromic microcytosis prolonged erythrocyte filtration times (EFT) were recorded; however this finding seemed better attributable to the larger filtered RBC number than to impaired erythrocyte deformability. The supposed influence of hypochromic microcytosis, due to repeated venesections, on blood …viscosity parameters in treated PV pts could not be confirmed. PV pts with “vascular risk” factors had higher values of WBV, plasma viscosity, and fibrinogen. It is concluded that: 1) hypochromic microcytosis is not per se associated with abnormal haemorheological behavior and 2) plasmatic factors greatly contribute to hyperviscosity in treated PV pts. Show more
Keywords: Polycythaemia Vera, blood viscosity, plasma viscosity, venesection, RBC filterability, MCV
DOI: 10.3233/CH-1986-6111
Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 1, pp. 99-108, 1986
Authors: Benner, Klaus-U. | Nordt, Frank J.
Article Type: Abstract
DOI: 10.3233/CH-1986-6112
Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 1, pp. 109-109, 1986
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