Clinical Hemorheology and Microcirculation - Volume 42, issue 1

Authors: Forconi, Sandro | Gori, Tommaso

Article Type: Other

DOI: 10.3233/CH-2009-1186

Citation: Clinical Hemorheology and Microcirculation, vol. 42, no. 1, pp. 1-6, 2009

Authors: Wang, Fang | Liu, Yu-Ying | Liu, Lian-Yi | Zeng, Qing-Jiang | Wang, Chuan-She | Sun, Kai | Yang, Ji-Ying | Guo, Jun | Fan, Jing-Yu | Han, Jing-Yan

Article Type: Research Article

Abstract: 3,4-dihydroxy-phenyl lactic acid (DLA) and salvianolic acid B (SAB) are two major water-soluble components of Salvia miltiorrhiza (SM). Previous works have revealed the ability of DLA and SAB to scavenge oxygen free radicals, inhibiting the expression of adhesion molecules CD11b/CD18 in neutrophil. Cardiotonic pills (CP), which is a traditional Chinese medicine compound preparation containing DLA and SAB, was found to inhibit venular thrombosis induced by photochemical reaction (PR) in rat mesentery. The present study addressed the effect of DLA and SAB on PR-induced thrombosis in rat mesentery by utilizing a microcirculation dynamic viewing system. The result demonstrated that both DLA …and SAB delayed thrombus-initiation time, while DLA also prolonged thrombus half-size time. The experiments explored the mechanism underlying that the dihydrorhodamine 123 (DHR) fluorescence in the mesenteric venular walls after PR challenge was diminished by pretreatment with either DLA or SAB, the expression of CD18 in neutrophils elicited by PR was depressed by administration of DLA, while mast cell degranulation in rat mesentery induced by PR was damped by SAB. The antioxidant potential of the two substances is likely to be responsible for their most beneficial effects on thrombosis, through either directly scavenging the peroxides produced and/or indirectly depressing the expression of adhesion molecules in neutrophil. Show more

Keywords: Antioxidant, adhesion molecule, mast cell degranulation, Salvia miltiorrhiza, thrombosis

DOI: 10.3233/CH-2009-1180

Citation: Clinical Hemorheology and Microcirculation, vol. 42, no. 1, pp. 7-18, 2009

Authors: Horvath, Beata | Szapary, Laszlo | Debreceni, Laszlo | Feher, Gergely | Kenyeres, Peter | Fulop, Adrienn | Battyani, Istvan | Toth, Kalman

Article Type: Research Article

Abstract: In our prospective study the effect of Sclerovit (0.8 mg folic acid, 20 μg vitamin B12 ,5 mg vitamin B6 ,100 mg vitamin E) on inflammatory markers, hemorheological parameters, platelet aggregation, von Willebrand factor activity as a marker of endothelium dysfunction, plasma lipids, plasma levels of folic acid, vitamin B12 and homocysteine (hcy), flow mediated vasodilatation (FMD) and thickness of carotis intima-media after 1 and 6 months of treatment in patients with vascular diseases (10 patients took 1 capsule, 10 patients 2 capsules of Sclerovit and 10 patients placebo) was determined. Plasma level of vitamin B12 , folic …acid and elongation index of red blood cells (RBC) increased significantly (p<0.05–0.001), hcy and triglyceride concentrations decreased significantly (p<0.05–0.001) in patients taking Sclerovit. HDL-cholesterol, RBC count, hematocrit, plasma and whole blood viscosity increased significantly (p<0.05–0.001) both in patients taking placebo or vitamins. Fibrinogen and CRP showed a significant (p<0.05–0.01) increase in patients on placebo, but did not change in patients on Sclerovit therapy. FMD showed a significant (p<0.05) amelioration in patients on 1 capsule of Sclerovit. Beside the favorable effects of Sclerovit on some of the measured parameters, the observed deterioration in hemorheological parameters can correlate with the contradictory results of large prospective studies with vitamins. Show more

Keywords: Homocysteine, endothelial dysfunction, hemorheological parameters, platelet aggregation, folic acid, vitamin B_6, vitamin B_12

DOI: 10.3233/CH-2009-1182

Citation: Clinical Hemorheology and Microcirculation, vol. 42, no. 1, pp. 19-28, 2009

Authors: Zheng, Jiao | Wang, Xianwei | Li, Haixia | Gu, Yueqing | Tu, Pengfei | Wen, Zongyao

Article Type: Research Article

Abstract: The aim of this study was to test the effects of Ilex kudingcha total saponins on hemorheology of ApoE-/- mice suffering from hypercholesterolemia induced by high-cholesterol diet. The mice were randomly divided into six groups: the control group, the high-cholesterol diet group, 50 mg/kg atorvastatin treatment group, 75, 150 and 300 mg/kg Ilex kudingcha saponins treatment groups, and all the drug treatment groups were fed with a high-cholesterol diet. After administration with saponins (150 mg/kg or more) and atorvastatin (50 mg/kg) for six weeks, the plasma total cholesterol (TC), whole blood viscosity (WBV), plasma viscosity (PV), and erythrocyte aggregation index …(EAI) had a remarkable decrease compared with that of the high-cholesterol diet group, but the hematocrit (Hct) and erythrocyte deformation index (DI) had no significant changes. In addition, it is found that the improving effects of saponins on reducing plasma fibrinogen (Fg) levels and prolonging the blood coagulation times including activated partial thromboplastin time (APTT), thrombin time (TT), and prothrombin time (PT). In conclusion, the Ilex kudingcha total saponins may have a significant therapy application of hypercholesterolemia and atherosclerosis by considering its actions on hemorheological characteristics. Show more

Keywords: Hypercholesterolemia, Ilex kudingcha total saponins, hemorheology

DOI: 10.3233/CH-2009-1183

Citation: Clinical Hemorheology and Microcirculation, vol. 42, no. 1, pp. 29-36, 2009

Authors: Bláha, M. | Rencová, E. | Bláha, V. | Malý, R. | Blažek, M. | Studnička, J. | Andrýs, C. | Fátorová, I. | Filip, S. | Kašparová, M. | Procházková, R. | Malý, J. | Zimová, R. | Langrová, H.

Article Type: Research Article

Abstract: Rheological conditions basically influence tissue perfusion, oxygen and nutrient supply, tissue regeneration and in its consequence the course of pathological processes, especially in microcirculation and partially even in macrocirculation. Haemorheotherapy has shown to be successful in several indications (critical disorders in microcirculation) when other methods of therapy have failed. In this study we describe the changes of effectivity indicators in haemorheopheresis treatment and their clinical importance in cases of eye microcirculation, statoacoustic apparatus and disorders of the lower extremities. We treated with haemorheopheresis (separator Cobe.Spetra + Evaflux filter) 24 patients (16 patients with age related macular degeneration, 5 with critical …peripheral arterial foot disease, and 3 with acute hearing loss). After the procedures α2 -macroglobulin decreases by about 58%, fibrinogen by about 69%, IgM by about 61%, LDL-cholesterol by about 77%, apolipoprotein B by about 76% and lipoprotein(a) by about 63%. It corresponds with a decrease in blood and plasma viscosity and clinical improvement in the observed patients, i.e. visual improvement, acceleration of tissue defect healing and improvement in hearing. We noticed 7.1% clinically insignificant side-effects. The method of haemorheopheresis was safe. It led to improvement in patients' clinical condition in the above mentioned indications when other methods of treatment failed. Show more

Keywords: Haemorheology, microcirculation, age related macular degeneration, diabetic foot, acute hearing loss

DOI: 10.3233/CH-2009-1184

Citation: Clinical Hemorheology and Microcirculation, vol. 42, no. 1, pp. 37-46, 2009

Authors: Musielak, M.

Article Type: Research Article

Abstract: Cell-deformability characterization involves general measurement of highly complex relationships between cell biology and physical forces to which the cell is subjected. The review takes account of the modern technical solutions simulating the action of the force applied to the red blood cell in macro- and microcirculation. Diffraction ektacytometers and rheoscopes measure the mean deformability value for the total red blood cell population investigated and the deformation distribution index of individual cells, respectively. Deformation assays of a whole single cell are possible by means of optical tweezers. The single cell-measuring setups for micropipette aspiration and atomic force microscopy allow conducting a …selective investigation of deformation parameters (e.g., cytoplasm viscosity, viscoelastic membrane properties). The distinction between instrument sensitivity to various RBC-rheological features as well as the influence of temperature on measurement are discussed. The reports quoted confront fascinating possibilities of the techniques with their medical applications since the RBC-deformability has the key position in the etiology of a wide range of conditions. Show more

Keywords: Diffraction ektacytometers, rheoscopes, atomic force microscopy, optical tweezers

DOI: 10.3233/CH-2009-1187

Citation: Clinical Hemorheology and Microcirculation, vol. 42, no. 1, pp. 47-64, 2009

Authors: López, María | Vayá, Amparo | Martínez Triguero, M. Luisa | Contreras, M. Teresa | Todolí, José | Ricart, Alicia | Laiz, Begoña

Article Type: Research Article

Abstract: It is not yet known whether Yasmin involves a higher thrombotic risk compared with other contraceptives. We present a serie of eight new cases of women who developed thrombotic events early after starting on Yasmin who were sent to our Thrombosis and Hemostasis Unit for a thrombophilia work-up in the last five years. Only two of them were heterozygous carriers of the prothrombin G20210A mutation and three were obese while none of them were smoker. These new cases provide information about the characteristics of the thrombotic events and the concomitant risk factors, indicating that this pill may not be as …safe as had been previously thought, and suggest that new studies regarding safety profile of Yasmin are required to explain the association with venous thrombotic events. Show more

Keywords: Yasmin, venous thromboembolism

DOI: 10.3233/CH-2009-1190

Citation: Clinical Hemorheology and Microcirculation, vol. 42, no. 1, pp. 65-69, 2009

Authors: Qi, Qixue | Li, Yufen | You, Yun | Liao, Fulong

Article Type: Other

DOI: 10.3233/CH-2009-1185

Citation: Clinical Hemorheology and Microcirculation, vol. 42, no. 1, pp. 71-73, 2009