Clinical Hemorheology and Microcirculation - Volume 3, issue 2

Authors: Milligan, D.W.

Article Type: Research Article

Abstract: Dilute suspensions of EDTA anticoagulated red blood cells from 32 patients with polycythemia vera were filtered through 5 µm polycarbonate filters in a constant flow system. When the buffy coat alone was removed the remaining white cells had a considerable influence on the filtration behaviour. A method was developed in which 95% of the white cells were removed by prefiltration of the whole blood through a natural cotton-wool filter. In further experiments the filterability of red cells from 19 polycythemic patients was compared with that from controls and found to show no significant difference. The small number of remaining white …cells still influenced the results and it is suggested that white cell artefacts may explain some of the difference previously reported by this technique. Show more

Keywords: Red cell filtration, leukocyte, polycythemia

DOI: 10.3233/CH-1983-3211

Citation: Clinical Hemorheology and Microcirculation, vol. 3, no. 2, pp. 155-162, 1983

Authors: Vanuxem, D. | Grimaud, Ch. | Guillot, Ch. | Gauthier, T. | Djian, I. | Monties, J.R.

Article Type: Research Article

Abstract: The authors realized from twelve patients with cardiac diseases, a kinetic study of the erythrocyte filtration flow (E.F.F) during and after extracorporeal circulation (E.C.C) surgery. In most of the cardiac patients, E.F.F was lower than in the control group; since the beginning of anesthesia, E.F.F increased and remained significantly higher throughout E.C.C with a maximal value at the 30th mn (2 p < 0.001). At the end of E.C.C and in the post-operative period, E.F.F decreased below the reference values (2 p < 0.001). In order to explain this E.F.F kinetic the authors discussed the influence of the following …factors: a) factors acting on the whole blood viscosity: hematocrit decrease and fibrinogen decrease enhanced the E.F.F; b) factors acting on the erythrocyte deformability: hypothermia and Pv¯O2 increase are positively correlated with E.F.F (2 p < 0.001); alcalosis is closely related to E.F.F decrease and there is a significant relationship (2 p < 0.05) between 2,3-DPG decrease and E.F.F decrease in the post-operative period (3th hour); the decrease of internal red cell viscosity, related to the mean corpuscular hemoglobin concentration (MCBC) enhanced erythrocyte deformability (2 p < 0.01). Total hemodilution seems to be important in explaining the E.F.F increase during E.C.C. It could also intervene in the post-operative period by limiting the 2,3-DPG decrease and so the E.F.F decrease. Show more

Keywords: Extracorporeal circulation, whole blood filtration, hypothermia, hemodilution, 2,3-Diphosphoglycerate

DOI: 10.3233/CH-1983-3212

Citation: Clinical Hemorheology and Microcirculation, vol. 3, no. 2, pp. 163-175, 1983

Authors: Nakache, M. | Caprani, A. | Dimicoli, J.L. | Massonnet, S. | Péronneau, P. | Jaulmes, B.

Article Type: Research Article

Abstract: The relation between deformability of RBCs and oxygen transfer is investigated by comparing RBCs from normal and Fallot donors. In the present modelization of the biological system, the flow is generated by a platinum disc electrode rotated, under laminar conditions, in a RBC suspension, and the oxygen transfer to the wall is measured by the rate of the electrochemical reduction of oxygen to water. Deformability, measured by filterability, is shown to be lower for Fallot than for normal RBCs. The results on oxygen reduction reveal that an increase in the deformability of the RBC gives rise to an increase of …oxygen transfer which corresponds mainly to an increase of the diffusion coefficient of oxygen in the suspending medium. Show more

Keywords: RBC, deformability, oxygen transfer, electrochemical method, filterability method

DOI: 10.3233/CH-1983-3213

Citation: Clinical Hemorheology and Microcirculation, vol. 3, no. 2, pp. 177-189, 1983

Authors: Kenny, M.W. | Meakin, M. | Stuart, J.

Article Type: Research Article

Abstract: A gravity filtration technique, using cotton wool from an Imugard IG500 filter, has been developed for removal of leucocytes and platelets from EDTA- or heparin-anticoagulated blood prior to studies of erythrocyte deformability. This technique removed 98.1% of leucocytes and 99.8% of platelets, without causing haemolysis, and was found to be superior to alternative methods of cell removal including centrifugation, column sedimentation, and filtration through cellulose. The erythrocytes recovered after filtration through cotton wool were representative of the original blood sample, as judged by cell size distribution, and there was no selective removal of reticulocytes, irreversibly sickled cells, or Heinz-body containing …cells. Show more

Keywords: Blood platelets, Erythrocytes, Leukocytes, Rheology

DOI: 10.3233/CH-1983-3214

Citation: Clinical Hemorheology and Microcirculation, vol. 3, no. 2, pp. 191-200, 1983

Authors: Goubran, F. | Khattab, A.K. | Saad, M.I. | Wattab, A.

Article Type: Short Communication

Keywords: Circulating platelet aggregates, thrombocythemia, gangrene

DOI: 10.3233/CH-1983-3215

Citation: Clinical Hemorheology and Microcirculation, vol. 3, no. 2, pp. 201-204, 1983

Authors: Benner, K-U.

Article Type: Abstract

DOI: 10.3233/CH-1983-3216

Citation: Clinical Hemorheology and Microcirculation, vol. 3, no. 2, pp. 205-210, 1983

Article Type: Announcement

DOI: 10.3233/CH-1983-3217

Citation: Clinical Hemorheology and Microcirculation, vol. 3, no. 2, pp. 211-213, 1983