Clinical Hemorheology and Microcirculation - Volume 17, issue 4

Authors: Liao, Fu‐long | Li, Bin

Article Type: Research Article

Abstract: Shear‐induced platelet aggregation (SIPA) plays an important role in vascular diseases. In this study, SIPA was generated by a computerized cone‐plate apparatus. Six compounds from Chinese herbal medicine were selected for in vitro and in vivo tests. Rabbit and rat were involved in the tests accordingly. Ligustrazine hydrochloridi appeared to be most effective for inhibition of SIPA. The inhibition rate of SIPA reached 44.2% (p<0.01 ) for an in vitro concentration of 1 mg/ml, and 46.1% (p<0.01 ) for an in vivo dose of 50 mg/kg.

Citation: Clinical Hemorheology and Microcirculation, vol. 17, no. 4, pp. 315-318, 1997

Authors: Singh, Megha | Kumaravel, M.

Article Type: Research Article

Abstract: Aggregation of erythrocytes requires prolonged interaction of cellular and plasma constituents similar to in vivo conditions. To achieve this and then to analyse this process in various clinical and laboratory conditions, a PC‐AT based system is developed. The erythrocyte suspension at 5% hematocrit in plasma is placed in a glass chamber, and the changes in laser transmitted intensity due to movement of the aggregates and erythrocytes in the path of the beam are sequentially recorded. From these data, aggregate size index, aggregate sedimentation time index, time required for completion of process and total number of fluctuations are calculated. From these, …two additional parameters – effective number of cells and effective sedimentation duration – are calculated. The results show that in leprosy the aggregation of erythrocytes is reduced. In in vitro studies due to the cholesterol‐enrichment of the erythrocyte membrane and treatment with pentoxifylline the aggregation of cells is increased whereas in disprin treated cells this is reduced compared to that of normal erythrocytes. Show more

Citation: Clinical Hemorheology and Microcirculation, vol. 17, no. 4, pp. 319-324, 1997

Authors: Miao, H. | Xue, Q.F. | Hu, Q.H. | Zhang, H. | Wang, L.R. | Niimi, H. | Zhuang, F.Y.

Article Type: Research Article

Abstract: In situ expression of ICAM‐1 and ICAM‐1 mRNA on the lung tissue of asthmatic rats was studied by immunohistochemical staining and in situ hybridization, respectively. The results showed that in normal rats ICAM‐1 expression was rare on the endothelium of pulmonary artery and vein, and on the bronchial and alveolar epithelium. The distribution of ICAM‐1 expression on the different part of lung tissue of asthmatic rats was similar to that of normal rats, but the level of ICAM‐1 expression was significantly increased on the endothelium of pulmonary artery (EPA) and vein (EPV), bronchial epithelium (BEP) and alveolar epithelium (AEP) compared …with those of normal and sensitized controls. The distribution and expression of ICAM‐1 mRNA on the different part of lung tissue of normal and asthmatic rats were similar to that of ICAM‐1 expression. In asthmatic rats, the expression of ICAM‐1 mRNA on AEP and EPV was increased significantly compared with those of normal and sensitized controls. It is concluded that the increase of ICAM‐1 expression on endothelium of pulmonary vessels, epithelium of broncheoli and alveoli may play an important role of inflammatory cell infiltration in asthmatic rats, and the increased expression of ICAM‐1 in asthmatic rats was caused by the increase of expression of ICAM‐1 mRNA. Show more

Keywords: ICAM‐1, ICAM‐1 mRNA, asthma, rat

Citation: Clinical Hemorheology and Microcirculation, vol. 17, no. 4, pp. 325-331, 1997

Authors: Rasia, R.J. | Valverde, J.R. | Gentils, M. | Cauchois, C. | Stoltz, J.‐F.

Article Type: Research Article

Abstract: Energy evolved from hemagglutination reaction or spent in dissociating erythrocyte agglutinates has been proved to be an excellent parameter for analyzing cell–cell interactions mediated by bridging molecules such as antibodies or lectins. We developed a new rheo‐optical method to estimate the energy of dissociation of red blood cell agglutinates. In a Couette shear field agglutinates can be dissociated until a suspension of monodispersed cells is obtained. Intensity of light backscattered by suspended agglutinates increases during their mechanical dissociation. Variation of backscattered light intensity correlates with the energy spent in the process. The adhesive energy of erythrocyte agglutination induced by lectins …has been estimated by applying this method. Two specific lectins (Dolichus Biflorus agglutinin and Ulex Europaeus agglutinin) and a new lectin obtained from Amarantus Cruentus seeds which specificity is unknown were studied. Results obtained in this work for Dolichus Biflorus lectin are comparable with values published by other authors. An asymptotic decrease of adhesive energy was observed when the mechanical dissociation was applied several times on the same sample. Our results suggest that the cell detachment is accompanied by the extraction of membrane receptors. This finding is consistent with results obtained by other authors. Show more

Keywords: Agglutinate, lectin, dissociation, energy, blood group

Citation: Clinical Hemorheology and Microcirculation, vol. 17, no. 4, pp. 333-340, 1997

Authors: Riha, P. | Liao, F. | Stoltz, J.‐F.

Article Type: Research Article

Abstract: The coagulation of whole blood resulting a blood clot is initiated in vitro for studying the effect of rouleaux formation on the kinetics of coagulation and clot formation. The clot formation is monitored in the rotational viscosimeter and in the erythroaggregometer using a light backscattered technique. The intensity of flow as well as hematocrit were changed in a way to determine a tendency in the effect of rouleaux size on the rate of coagulation. The results indicate that the clot formation is promoted when size of rouleaux is small at higher shear rates and low hematocrit in comparison with the …coagulation at low shear rates and high hematocrit when the size of rouleaux is increased. Show more

Keywords: Blood coagulation, erythrocytes, hematocrit, flow intensity

Citation: Clinical Hemorheology and Microcirculation, vol. 17, no. 4, pp. 341-346, 1997

Article Type: Other

Citation: Clinical Hemorheology and Microcirculation, vol. 17, no. 4, pp. 347-347, 1997