Affiliations: [a] Laboratoires de Biophysique, UER Blomédicale, 45 rue des Salnts-Pères, 75270-Parls Cedex 06, et UER de Médecine, 74 rue Marcel Cachin, 93000-Boblgny, France | [b] Laboratoire de Biologie Cellulaire, UER de Médecine, 74 rue Marcel Cachln, 93000-Boblgny, France
Note: [] Accepted by: Editor M.R. Boisseau
Abstract: The effect of sialic acid of erythrocytes on the cellular aggregation and deformability was quantitatively examined. Desialylated red blood cells were obtained after incubation with neuraminidase at different concentrations. The electrophoretic mobility was controlled. Erythrocyte aggregation was measured via ultrasound backscattering on erythrocytes resuspended In autoloqous plasma. Red blood cell deformability was studied on dilute suspensions In phosphate buffer by the Initial flow rate filtration method and the cell transit time analysis. For desialylated red blood cells In suspension. Aggregation is increased due to the reduction of the electrostatic repulsive force among erythrocytes. An increase of the cellular transit time is found. A reason which could explain this phenomenon is the role of the cell membrane-pore wall sieves Interactions.
