Affiliations: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong | Department of Biology, Hong Kong Baptist University, Hong Kong | Division of Cardiology, Department of Medicine, Tokai University School of Medicine, Kanagawa, Japan | Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China | Institute of Integrated Traditional Chinese and Western Medicine, Sun Yat‐Sen University, Guangzhou 510080, China
Note: [] Corresponding author: Dr. Min Li, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong. E‐mail: limin@hkbu.edu.hk.
Abstract: Tetramethylpyrazine (TMP) and salvianolic acid B (SAB) are effective ingredients of Rhizoma Ligustici chuanxiong Hort. and Radix Salviae miltiorrhizae Bge., accordingly. The inhibitive effects of TMP, SAB and their combination on shear‐induced platelet aggregation (SIPA) were investigated in the present study. SD rats were used as blood donors to collect anticoagulated blood, the concentration of platelet‐rich‐plasma (PRP) was adjusted to 5×105 μl. HAAKE rheometer RS 600 with sensor C60/0.5° was used as shear generator. Different doses of TMP and SAB and their combinations were added to the PRP. After constant shear of 15 Pa at 37°C for 360 seconds, PRP was transferred to a platelet aggregometer and SIPA was determined by turbidity. SIPA was inhibited by TMP and SAB in a dose‐dependent manner. SIPA was decreased from 48.6±4.6% of the control to 12.5±2.1% in the presence of TMP (1.46 mM) and SAB (10 μM) (P<0.0001). In conclusion, TMP and SAB have additive effects on inhibiting platelet aggregation induced by high shear stress.
Keywords: Tetramethylpyrazine (TMP), salvianolic acid B (SAB), platelet aggregation, shear stress, Chinese Medicine Partner (CMP)
