Affiliations: [a] Osaka City University Medical School, Department of Laboratory Medicine, Abeno, Osaka, Japan | [b] Osaka City University Medical School, Department of Physiology, Abeno, Osaka, Japan
Abstract: In order to clarify the cause of a high variation of hematocrit values, the effects of undeformable red cells on trapped plasma volume were studied. The volume was determined for [Vettore, L., Falezza, G., Demateus, M.C., Cetto, G. and Zandeziacoma, M. A new method for the determination of sodium and pottasium in human red blood cells using indocyanine green as a marker for trapped plasma. Clinica chimica Acta, 55, 345–351, 1974] the mixture of normal cells and undeformable cells which had been prepared by glutaraldehyde fixation and [International committee for standardization in hematology expert panel on blood cell sizing. Recommendation for reference method for determination by centrifugation of packed cell volume of blood. J. Clin. Pathol. 33, 1–2, 1980] heated cells from 46 to 50°C for 10 minutes. Trapped plasma volume increased exponentially with the increase in the number of undeformable cells. Subpopulation of completely undeformable cells fixed with glutaraldehyde in the cell suspension augmented the amount of trapped plasma volume in the microhematocrit as compared with that of a whole population of cells heated at elevated temperature. The findings indicated that marginally reduced deformability may lead to spurious values for the hematocrit using spun hematocrit.
