Affiliations: Laboratoire d'Angiohématologie‐Hémorhéologie, UMR CNRS 7563 (LEMTA), Faculté de Médecine, 54500 Vandoeuvre‐lès‐Nancy Cedex, France | Laboratoire de Physico‐chimie des Colloïdes, UMR CNRS 7565 (SRSMC), Faculté des Sciences, U.H.P. Nancy 1, BP 239, 54506 Vandoeuvre‐lès‐Nancy Cedex, France
Note: [] Corresponding author: J. Didelon, CAV, Laboratoire IMAC, Avenue de Bourgogne, 54511 Vandoeuvre Cédex, France. Tel.: +33 3 83 59 84 85; E‐mail: j.didelon@nancy.fnclcc.fr.
Abstract: The aim of the present work was to validate a new technique for the measurement of resistance of the red blood cell membrane using an automated apparatus called a Fragilimeter. Its principle lies in the measurement of the extinction of a laser beam projected through a red blood cell suspension subjected, by diffusion, to a variation of salinity from an isotonic equilibrium (154 mM NaCl) to, a hypotonic one, 25 mM NaCl. The variation of osmotic pressure induces on the cells a progressive lysis and a modification of the extinction of the transmitted light. The validation of the method was based on the comparison between results obtained with the Fragilimeter and those obtained using the reference DACIE technique. Analyses were based on blood samples from healthy donors. The determination of the initial, the 50% and the full haemolysis thresholds allowed observation of the fragility of the cell, through its membrane resistance. The physical phenomenon measured in these cells when subjected to various ionic strengths is discussed on the basis of observations realised by means of an optical microscope.
Keywords: Red blood cells, osmotic resistance, fragility, haemolysis, deformability
