Affiliations: [a] Faculty of Physics, M.V. Lomonosov Moscow State University, Moscow, Russia
| [b]
K.D. Ushinsky Yaroslavl State Pedagogical University, Yaroslavl, Russia
| [c]
N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Moscow, Russia
Correspondence:
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Corresponding author: Petr Ermolinskiy, Faculty of Physics, M.V. Lomonosov Moscow State University, Moscow, Russia. Tel.: +7 915 114 87 59; E-mail: ermolinskiy.pb15@physics.msu.ru.
Abstract: Blood microrheology depends on the constituents of blood plasma, the interaction between blood cells resulting in red blood cell (RBC) and platelets aggregation, and adhesion of RBC, platelets and leukocytes to vascular endothelium. The main plasma protein molecule –actuator of RBC aggregation is fibrinogen. In this paper the effect of interaction between the endothelium and RBC at different fibrinogen concentrations on the RBC microrheological properties was investigated in vitro. Laser tweezers were used to measure the RBC-endothelium interaction forces. It was shown for the first time that the interaction forces between RBC and endothelium are comparable with the RBC aggregation forces, they increase with fibrinogen concentration and reach the saturation level of about 4 pN at the concentration of 4 mg/ml. These results are important for better understanding the mechanisms of RBC and endothelium interaction and developing the novel therapeutic protocols of the microrheology correction in different pathologies.
Keywords: Red blood cells, endothelium, fibrinogen, laser tweezers, red blood cell, aggregation, adhesion
