Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Issue title: Selected post-conference papers of the 39th Conference of the German Society for Clinical Microcirculation and Hemorheology, 6-7 November 2020, Hannover, Germany
Article type: Research Article
Authors: Monteleone, Adriana; b | Brandau, Lenaa; * | Schary, Weronikaa | Wenzel, Folkera
Affiliations: [a] Faculty of Medical and Life Sciences, Hochschule Furtwangen, Villingen-Schwenningen, Germany | [b] Human and Environmental Toxicology, University Konstanz, Constance, Germany
Correspondence: [*] Corresponding author: Lena Brandau, Faculty of Medical and Life Sciences, Hochschule Furtwangen, Villingen-Schwenningen, Germany. E-mail: wfo@hs-furtwangen.de.
Note: [1] This article was presented at the 39th Conference of the German Society for Clinical Microcirculation and Hemorheology, 6-7 November 2020, Hannover, Germany.
Abstract: INTRODUCTION:More and more researchers are studying the effects of microplastics on the environment and the organisms living in it. Existing detection methods still require a heavy workload, complex sample preparation and high costs. In this study, autofluorescence of plastic was used as a new method for microplastic detection. MATERIAL AND METHODS:Particles of common plastics were incubated at various temperatures (21–230 °C) for different time periods to investigate the influence of these conditions on their autofluorescence using methods like fluorescence microscopy, and measurement of absorption and emission. To give an example of an autofluorescence application, ImageJ was used to determine the contamination of microplastic in sea salt samples. RESULTS:After treatment at 140 °C for 12 h the plastics ABS, PVC and PA showed a distinct increase in their fluorescence intensity. For PET higher temperatures were necessary to achieve higher fluorescence intensities. Using ImageJ, the particle contamination in sea salt samples was determined as 4903±2522 (aluminium membrane) / 5053±2167 (silicone membrane) particles in 10 g salt, which is a much higher number than counted in other publications. DISCUSSION:Probably the increase in fluorescence intensity is due to the movement of atomic bonds caused by the thermic energy during the heat treatment. The high number of counted particles by using ImageJ is most likely based on the smaller pore size of the used filter membranes and other contaminations like dust and fibers, which could be avoided by alternative sample treatment. CONCLUSION: Considering the outcomes of this study, heat treatment is a useful tool to make microplastic particles more visible in microscopic applications without readable destruction of their composition. The heat treatment of plastics for defined incubation times and temperatures can lead to a distinct increase in autofluorescence intensity of the plastics and therefore serve as an easy and cost-effective applicable method for microplastic detection.
DOI: 10.3233/CH-209223
Journal: Clinical Hemorheology and Microcirculation, vol. 76, no. 4, pp. 473-493, 2020
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl