Arthrospira platensis accelerates the formation of an endothelial cell monolayer and protects against endothelial cell detachment after bacterial contamination

Article type: Research Article

Authors: Krüger-Genge, A.^{a; *} | Steinbrecht, S.^b | Jung, C.G.H.^b | Westphal, Sophia^a | Klöpzig, Stefanie^a | Waldeck, P.^b | Küpper, J.-H.^{b; c} | Storsberg, J.^a | Jung, F.^b

Affiliations: [a] Fraunhofer Institute for Applied Polymer Research (IAP), Potsdam-Golm, Germany | [b] Institute of Biotechnology, Molecular Cell Biology, Brandenburg University of Technology, Senftenberg, Germany | [c] Carbon Biotech, Social Enterprise Stiftungs AG, Senftenberg, Germany

Correspondence: [*] Corresponding author: A. Krüger-Genge, Fraunhofer Institute for Applied Polymer Research (IAP), Potsdam-Golm, Germany. E-mail: anne.krueger-genge@iap.fraunhofer.de.

Abstract: Within the last years a comprehensive number of scientific studies demonstrated beneficial effect of Arthropira platensis (AP) as dietary supplement due to a high content of proteins, minerals and vitamins. Positive effects like promoting the immune system, reducing inflammation and an anti-oxidant capacity are reported. In this study, the effect of an aqueous AP extract on primary human venous endothelial cells (HUVEC) was investigated. In addition, the effect of AP on HUVEC treated with a bacterial toxin (lipopolysaccharide, LPA), inducing an activation of HUVEC and cellular detachment, was analyzed. Depending on the concentration of AP extract a significantly accelerated formation of an endothelial cell monolayer was observed. Furthermore, the detachment of HUVEC after LPA addition was dramatically reduced by AP. In conclusion, the data are promising and indicatory for an application of Arthrospira platensis in the clinical field.

Keywords: Arthrospira platensis, endothelial cells, xCELLigence system

DOI: 10.3233/CH-201096

Journal: Clinical Hemorheology and Microcirculation, vol. 78, no. 2, pp. 151-161, 2021