Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Chen, Ji-Xionga; 1 | Huang, Xiao-Yanb; 1 | Wang, Pinga | Lin, Wen-Tinga | Xu, Wen-Xinga | Zeng, Mina; *
Affiliations: [a] Department of Medical Care Center, Hainan Provincial People’s Hospital, Haikou, China | [b] Department of Pediatrics, Hainan Maternal and Child Health Hospital, Haikou, China
Correspondence: [*] Corresponding author: Min Zeng, Department of Medical Care Center, Hainan Provincial People’s Hospital. No.19, Xiuhua Road, Xiuying District, Haikou 570311, China. Tel.:+86 0898 68622312; Fax:+86 0898 6862372; mzdr_sea06@163.com.
Note: [1] These authors contributed equally to this study.
Abstract: This study aimed to investigate the effects of arachidonic acid metabolite epoxyeicosatrienoic acid (EETs) in the apoptosis of endothelial cells induced by tumor necrosis factor-alpha (TNF-α). After human umbilical vein endothelial cells were cultured, TNF-α/ActD, 14, 15-EET, and HMR-1098 were added, respectively, into the culture medium. The apoptosis level of endothelial cells was detected by flow cytometry. After TNF-α/ActD induced endothelial cell apoptosis, flow cytometry staining showed that endothelial cell apoptosis increased significantly, and the apoptotic cells were significantly reduced after the addition of 14, 15-EET. However, the apoptotic cells significantly increased after the addition of HMR-1098. Western Blot results showed that the phosphorylation levels of LC3-II and AMPK were increased after TNF-α/ActD induction, and the increase was noticeable after the addition of 14, 15-EET. However, the phosphorylation levels of LC3-II and AMPK significantly decreased after the addition of HMR-1098. The activity of Caspase-8 and -9 decreased significantly after the addition of 14, 15-EET but increased after the addition of HMR-1098. Arachidonic acid can inhibit TNF-α induced endothelial cell apoptosis by upregulating autophagy.
Keywords: Epoxyeicosatrienoic acid, autophagy, apoptosis, endothelial cell, TNF-α
DOI: 10.3233/CH-200946
Journal: Clinical Hemorheology and Microcirculation, vol. 77, no. 3, pp. 259-265, 2021
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl