Impact of platelet-rich plasma on cell migration processes after external radiation

Issue title: Selected papers of the 38th Conference of the German Society for Clinical Microcirculation and Hemorheology, 21-23 November 2019, Braunschweig, Germany

Guest editors: P. Wiggermann, A. Krüger-Genge and F. Jung

Article type: Research Article

Authors: Reinders, Yvonne^{a; b; *} | Pohl, Fabian^c | Ahrens, Norbert^d | Prantl, Lukas^a | Kuehlmann, Britta^a | Haubner, Frank^{e; *}

Affiliations: [a] Center for Plastic, Aesthetic, Hand & Reconstructive Surgery, University Hospital Regensburg, Regensburg, Germany | [b] Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V., Dortmund, Germany | [c] Department of Radiotherapy, University Hospital Regensburg, Regensburg, Germany | [d] Department of Clinical Chemistry and Laboratory Medicine, Transfusion Medicine, University Hospital Regensburg, Regensburg, Germany | [e] Department of Otorhinolaryngology, Ludwig-Maximilians-University Munich, Munich, Germany

Correspondence: [*] Corresponding author: Frank Haubner, MD, Department of Otorhinolaryngology, Ludwig-Maximilians-University Munich, 81377 Munich, Germany. E-mail: Frank.Haubner@med.uni-muenchen.de and Yvonne Reinders, PhD, Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V. 44227 Dortmund, Germany. E-mail: yvonne.reinders@isas.de.

Abstract: BACKGROUND:To overcome the compromised wound healing in radiation induced chronic wounds platelet-rich plasma (PRP), as therapeutic agent, is current subject of studies. PRP is associated with pro-angiogenic effects. Nevertheless, effects of platelet-rich plasma in cutaneous wound healing processes are poorly understood so far. Methods:In this study, the migration of endothelial cells, fibroblasts and keratinocytes in conjunction with platelet-rich plasma treatment is investigated in the context of radiation effects. Additionally, cell proliferation and viability after external radiation was analyzed regarding treatment by platelet-rich plasma. RESULTS:All cell cultures showed a trend towards decreasing proliferation and viability after irradiation irrespective of PRP. Upon PRP treatment, irradiated fibroblasts as well as endothelial cells showed an enhanced proliferation whereas proliferation and viability of keratinocytes was reduced after PRP treatment. Scratch assays support the positive effect of PRP on fibroblast and endothelial cell migration, whereas a negative effect on keratinocytes was observed after PRP treatment. CONCLUSIONS:The present study documents both deleterious effects of external radiation as well as the protective effect of PRP. In summary, increased viability, proliferation and migration are indeed a consequence of the pro-proliferative effect exerted by PRP. Therefore, treatment with PRP products might be useful in the management of chronic radiogenic wounds.

Keywords: Wound healing, irradiation, platelet-rich plasma, scratch assay, viability, proliferation

DOI: 10.3233/CH-199218

Journal: Clinical Hemorheology and Microcirculation, vol. 73, no. 1, pp. 43-51, 2019