Measurement of platelet aggregation functions using whole blood migration ratio in a microfluidic chip

Article type: Research Article

Authors: Seo, Hong Seog^a | Choi, Sung Hyuk^b | Han, Miran^c | Kim, Kyeong Ah^c | Cho, Chi Hyun^c | An, Seong Soo A.^d | Lim, Chae Seung^{c; *} | Shin, Sehyun^{e; *}

Affiliations: [a] KU-KIST Graduate School of Converging Science and Technology, Seoul, Korea | [b] Department of Emergency Medicine, College of Medicine, Korea University, Guro Gu, Seoul, Korea | [c] Department of Laboratory Medicine, College of Medicine, Korea University, Guro Gu, Seoul, Korea | [d] College of Bionano Technology, Gachon Bionano Research Institute, Gachon University, Seongnam-si, Gyeonggi Do, Korea | [e] School of Mechanical Engineering, Korea University, Seoul, Korea

Correspondence: [*] Corresponding author: Chae Seung Lim, MD, Department of Laboratory Medicine, Guro Hospital, Korea University College of Medicine, Guro 2 Dong, Guro Gu, Seoul 152-703, Korea. Tel.: +82 2 2626 3245; Fax: +82 2 2626 1465; E-mail: malarim@korea.ac.kr.

Correspondence: [*] Corresponding author: Sehyun Shin, Ph.D., School of Mechanical Engineering, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Korea. Tel.: +82 2 3290 3377; E-mail: lexerdshin@korea.ac.kr.

Abstract: Platelets play a major role in maintaining endothelial integrity and hemostasis. Of the various soluble agonists, ADP is an important in vivo stimulus for inducing platelet aggregation. In this study, a simple, rapid, and affordable method was designed for testing bleeding time (BT) and platelet aggregation with a two-channel microfluidic chip. Whole blood migration ratio (MR) from a microchip system was evaluated in comparison to the closure time (CT) from PFA-100 assays (Siemens, Germany) and CD62P expression on platelets. To induce platelet aggregation, a combination of collagen (1.84 mg/ml) and ADP (37.5 mg/ml) were used as agonists. After adding the agonists to samples, whole blood MR from the microchip system was measured. The outcome of the assessment depended on reaction time and agonist concentration. MR of whole blood from the microchip system was significantly correlated with CT from PFA-100 (r = 0.61, p <  0.05, n = 60). In addition, MR was negatively correlated with CD62P expression (r =−0.95, p <  0.05, n = 60). These results suggest that the measurement of MR using agonists is an easy, simple and efficient method for monitoring platelet aggregation in normal and ADP-receptors defective samples, along with the BT test. Thus, usage of the current microfluidic method could expand to diverse applications, including efficacy assessments in platelet therapy.

Keywords: Platelet, ADP, Collagen, PFA-100

DOI: 10.3233/CH-151961

Journal: Clinical Hemorheology and Microcirculation, vol. 62, no. 2, pp. 151-163, 2016