Affiliations: [a]
Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany
| [b] Department of Cardiology, Campus Virchow Klinikum, Charité, Berlin, Germany
| [c] Institute for Veterinary Pathology, Freie Universität Berlin, Berlin, Germany
| [d]
Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany
| [e] Evonik Creavis GmbH, Marl, Germany
Correspondence:
[*]
Corresponding author: Tobias Haase, Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany. E-mail: tobias.haase@charite.de.
Abstract: Copolyetheresterurethane (PDC) is a biodegradable, shape-memory biomaterial, which has been shown to be of low toxicity and pro-angiogenic in vitro. In the present study we examined the in vivo compatibility of PDC as a compression molded film and as electrospun scaffolds and its well established constituent, the homopolymer poly(p-dioxanone) (PPDO), which were compared with the clinically used poly[(vinylidene fluoride)-co-hexafluoropropene] (PVDF) as reference material. The materials were implanted in the subcutaneous tissue of mice and the host responses were analyzed histologically 7 and 28 days after implantation. All materials induced a foreign body response (FRB) including the induction of foreign body giant cells and a peripheral fibrous capsule. PDC, PPDO and PVDF films showed no signs of degradation after 28 days. PDC films showed a significantly reduced associated macrophage layer and fibrous capsule on their surface. Few fragments of PDC and PPDO scaffolds were present at the implantation site, while PVDF scaffolds were still present in large amounts at day 28. Especially aligned electrospun PDC scaffold induced a significantly thinner fibrous and a slightly reduced inflammatory response after 28 days of implantation. In addition, only PDC aligned fibrous scaffold structures induced a significant increase in angiogenesis. In summary, PDC films outperformed PPDO and PVDF films in terms of compatibility, especially in capsule and macrophage layer thickness. Through microstructuring of PDC and PPDO into scaffolds an almost complete degradation was observed after 28 days, while their respective films remained almost unchanged. However, the capsule thickness of all scaffolds was comparable to the films after 28 days. Finally, the parallel arrangement of PDC fibers enabled a strong enhancement of angiogenesis within the scaffold. Hence, material chemistries influence overall compatibility in vivo, while angiogenesis could be influenced more strongly by microstructural parameters than chemical ones.
Keywords: Degradable polymer, electrospinning, scaffold, microstructure, in
vivo compatibility, foreign body reaction, neovascularization, tissue integration, shape-memory
polymer, homopolymer, copolymer
