Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Carelli-Alinovi, Cristianab | Pirolli, Davideb | Giardina, Brunob; c | Misiti, Francescoa; *
Affiliations: [a] Human, Social and Health Department, University of Cassino and Southern Lazio, V.S. Angelo, Loc. Folcara, Cassino (FR), Italy | [b] Biochemistry and Clinical Biochemistry Institute, Catholic University, School of Medicine, Rome, Italy | [c] Istituto di Chimica del Riconoscimento Molecolare (ICRM), National Research Council (CNR), Rome, Italy
Correspondence: [*] Correspondence to: Francesco Misiti, Department of Human Sciences, Society and Health University of Cassino and Southern Lazio, Italy V.S. Angelo-Polo didattico della Folcara Cassino (FR) 03043, Italy. Tel.: +39 7762994423; f.misiti@unicas.it
Abstract: We have previously showed that morphological alterations in Red Blood Cells (RBCs) are correlated to an impaired eNOS enzymatic activity and a concomitant reduced NO derived metabolites formation. Here we extend our previous observations, reporting that RBC morphology is regulated by a series of specific cell signaling events linked to Protein Kinase C (PKC)-mediated activation of caspase 3. Pretreatment of RBCs with the PKC inhibitor chelerythrine, prior to the addition of phorbol-12-myristate-13-acetate (PMA), an activator of PKC, blocks the appearance of the morphology alterations and the sustained decrease in nitrates and nitrites levels induced by PMA. Inhibition of PKC also completely inhibits PMA mediated caspase-3 activation. On the other hand, caspase 3 inhibition, lessens the PMA induced-effects on the appearance of RBC morphology alterations, although it enhances PMA-mediated effects on nitric oxide (NO) derived metabolites levels. These data demonstrate that PKC-mediated activation of caspase 3 is an integral and essential part of signaling pathway in RBCs, that may be a regulatory factor of RBC mechanical properties, through regulation of NO metabolism.
Keywords: RBCs, nitric oxide, PKC, caspase 3, phorbol-12-myristate-13-acetate
DOI: 10.3233/CH-141845
Journal: Clinical Hemorheology and Microcirculation, vol. 59, no. 4, pp. 345-354, 2015
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl