Beneficial effects of remote organ ischemic preconditioning on micro-rheological parameters during liver ischemia-reperfusion in the rat

Article type: Research Article

Authors: Magyar, Zsuzsanna^a | Mester, Anita^a | Nadubinszky, Gabor^a | Varga, Gabor^a | Ghanem, Souleiman^a | Somogyi, Viktoria^a | Tanczos, Bence^a | Deak, Adam^a | Bidiga, Laszlo^b | Oltean, Mihai^{c; d} | Peto, Katalin^{a; 1} | Nemeth, Norbert^{a; 1; *}

Affiliations: [a] Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary | [b] Department of Pathology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary | [c] The Transplantation Institute, Sahlgrenska University Hospital, Gothenburg, Sweden | [d] Department of Surgery, Institute for Clinical Sciences, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden

Correspondence: [*] Corresponding author: Norbert Nemeth, MD, PhD, DSc, Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Nagyerdei krt. 98., Hungary. Tel./Fax: +36 52 416 915; E-mail: nemeth@med.unideb.hu.

Note: [1] These authors are contributed equally this work.

Abstract: BACKGROUND:Remote ischemic preconditioning (RIPC) can be protective against the damage. However, there is no consensus on the optimal amount of tissue, the number and duration of the ischemic cycles, and the timing of the preconditioning. The hemorheological background of the process is also unknown. OBJECTIVE:To investigate the effects of remote organ ischemic preconditioning on micro-rheological parameters during liver ischemia-reperfusion in rats. METHODS:In anesthetized rats 60-minute partial liver ischemia was induced with 120-minute reperfusion (Control, n = 7). In the preconditioned groups a tourniquet was applied on the left thigh for 3×10 minutes 1 hour (RIPC-1, n = 7) or 24 hours (RIPC-24, n = 7) prior to the liver ischemia. Blood samples were taken before the operation and during the reperfusion. Acid-base, hematological parameters, erythrocyte aggregation and deformability were tested. RESULTS:Lactate concentration significantly increased by the end of the reperfusion. Erythrocyte deformability was improved in the RIPC-1 group, erythrocyte aggregation increased during the reperfusion, particularly in the RIPC-24 group. CONCLUSIONS:RIPC alleviated several hemorheological changes caused by the liver I/R. However, the optimal timing of the RIPC cannot be defined based on these results.

Keywords: Ischemia-reperfusion, remote ischemic preconditioning, hemorheology

DOI: 10.3233/CH-170351

Journal: Clinical Hemorheology and Microcirculation, vol. 70, no. 2, pp. 181-190, 2018