Affiliations: [a] Department of Biological Sciences in Sport, Faculty of Sports Sciences and Health, Shahid Beheshti University, Iran
| [b] Cell-Based Therapies Research Center, Digestive Disease Research Institute, Tehran University of Medical Science, Tehran, Iran
Correspondence:
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Corresponding author: Dr. Sajad Ahmadizad, Department of Biological Sciences in Sport, Faculty of Sports Sciences and Health, Shahid Beheshti University, Velenjak, 1983963113, Tehran, Iran. Tel.: +98 21 29902931; Fax: +98 21 22431953; E-mail: S_ahmadizad@sbu.ac.ir.
Abstract: BACKGROUND:Type 2 diabetes mellitus (T2DM) is associated with thrombotic events due to platelets’ defects. OBJECTIVE:The aim of this study was to investigate the effects of endurance training on expressions of platelet hsa-miR-223 and P2RY12 receptor, as well as platelet function in T2DM patients. METHODS:Twenty female T2DM patients (age, 62.25±3.81 yr; weight, 73.53±9.04 kg; height, 154.7±4.3 cm) were randomly divided into two groups (Control (CONT) and endurance training (ET)). Subjects in ET group performed eight weeks of running on treadmill at 60–75% of VO2peak (moderate intensity), 3 sessions per week, while, the subjects in control group were not involved in any kind of exercise training. Two blood samples were taken before and 48 h after the training and were analyzed for platelet aggregation, and hsa-miR-223 and P2RY12 expressions. RESULTS:Although platelet aggregation decreased significantly in ET group (P < 0.05), these changes were not significantly different between two groups. Expression of platelet hsa-miR-223 increased and P2RY12 mRNA reduced following ET non-significantly. However, decreases in fasting blood glucose, glycated hemoglobin and body weight, and increases in VO2peak following ET were significantly different when compared to control group (P < 0.05). CONCLUSIONS:Short-term endurance training dose not induce up-regulation of hsa-miR-223 and down-regulation of P2RY12, while it has a positive impact on platelet function, glycemic indices, physical fitness and body composition in female T2DM patients.
