Platelet membrane fluidity, platelet membrane lipid pattern and platelet cytosolic Ca²⁺ content in subjects with vascular atherosclerotic disease

Article type: Research Article

Authors: Caimi, G. | Lo Presti, R. | Montana, M. | Canino, B. | Ventimiglia, G. | Catania, A. | Sarno, A.

Affiliations: Istituto di Clinic a Medica e Malattie Cardiovascolari, Universita' degli Studi di Palermo, Via del Vespro 129, 90127 Palermo, Italy

Abstract: In a group of subjects with vascular atherosclerotic disease (VAD) we examined the platelet membrane fluidity (obtained marking intact resting platelets with TMA-DPH), the platelet membrane cholesterol/phospholipid ratio (C/PL - using column chromatography), the platelet membrane individual phospholipids (employing the thin layer chromatography) and the platelet cytosolic Ca2+ content (evaluated marking intact resting platelets with Fura 2-AM). From the obtained data, it is evident that platelet membrane fluidity differentiates normals from VAD subjects. Platelet membrane lipid pattern (C/PL and individual phospholipids) and cytosolic Ca2+ content do not discriminate normals from VAD subjects. In normals the polarization degree of TMA-DPH is significantly correlated to the phosphatidylserine and phosphatidylcholine, while in VAD subjects no relationship is present between the polarization degree of TMA-DPH and the platelet metabolic parameters.

Keywords: Vascular Atherosclerotic Disease, Platelet Membrane Fluidity, Platelet Membrane Lipids, Platelet Cytosolic Ca2+

DOI: 10.3233/CH-1994-14309

Journal: Clinical Hemorheology and Microcirculation, vol. 14, no. 3, pp. 393-399, 1994