Evaluation of platelet aggregability by computerised probit analysis

Article type: Research Article

Authors: Mukherjee, Manjari | Desai, Amita P.

Affiliations: Sir H. N. Medical Research Society, Raja Rammohan Roy Road, Bombay 400 004, India

Abstract: The concentration of 5'-adenosine diphosphate (ADP) required to produce fifty percent of the estimated maximal response (ADP EC50) was determined by computerised probit analysis. This correlated directly and significantly with the minimum concentration of ADP required to produce (i) a biphasic aggregation response (ADPBiphasic); and (ii) a maximal aggregation response exceeding 50% (ADPPA > 50). Platelet aggregability was evaluated in 70 subjects consisting of 18 patients with coronary heart disease (CHD), 13 with ischemic cerebrovascular disease (CVD), 18 with rheumatoid arthritis (RA) and in 21 healthy controls. The mean (SEM) values of EC50 were slightly but not significantly decreased (hence platelet aggregability increased) in patients with CHD (2.58 +− 0.36 μmoles/L) and RA (2.23 +− 0.36 μmoles/L) as compared with controls (2.94 +− 0.34 μmoles/L); and slightly elevated in patients with ischemic CVD (3.39 +− 0.34 μmoles/L). The mode values of ADPPA > 50 were also less in the CHD and RA groups (2 μmoles/L) as compared with the controls (4 μmoles/L); in the CVD group the mode was 4 μmoles/L. The mode values of ADPBiphasic were however the same in all the four groups, namely 2 μmoles/L.

Keywords: Probit analysis, platelet aggregation

DOI: 10.3233/CH-1994-14208

Journal: Clinical Hemorheology and Microcirculation, vol. 14, no. 2, pp. 223-231, 1994