Affiliations: Department of Pharmacology, Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing, 100700, China
Abstract: Based on rotational viscometry of clotting, an approach for evaluation of antiplatelet drugs and anticoagulants was explored. Rat platelet rich plasma (PRP) and platelet poor plasma (PPP) were prepared. PRP and PPP were seperately incubated with and without drug (D), namely, PRP, PRP+D, PPP, and PPP+D. Recalcification of the samples were monitored by HAAKE Rotovisco RV20/CV100 at 0.3 s−1. The inhibition of platelet involvement (IPI) and the inhibition of plasma clotting (IPC) were defined. According to three clotting parameters (tr, dη/dt, τm), six inhibitions (3 IPI, 3 IPC) were obtained for evaluating a drug. The preliminary investigation on heparin, pentoxifylline, aspirin, notoginseng saponins and alcohol indicated that the approach would be a promising mean for pharmacological and clinical practice.
