Application of Laser-Doppler electrophoresis to the measurement of blood cell surface charge

Article type: Research Article

Authors: Janot, C.^{a; b} | Muller, S.^b | Streiff, F.^{a; b} | Donner, M.^b | Stoltz, J.F.^{a; b}

Affiliations: [a] Centre Régional de Transfusion Sanguine de Nancy, Avenue de Bourgogne, 54500 Vandoeuvre les Nancy, France | [b] Unite INSERM 284 CO 10 Brabois, 54511 Vandoeuvre les Nancy, France

Note: [] Accepted by: Editor M.R. Boisseau

Abstract: The surface charge of human erythrocytes and its variations in different experimental conditions were analysed by a Laser-Doppler velocimetry technique. The mean electrophoretic mobility of human erythrocytes was determined in relation to the suspending medium in which ionic strength, pH and viscosity varied. The influence of some proteolytic enzymes (bromelase, neuraminidase) as well as that of AIHA auto-antibodies on electrophoretic mobility was assessed according to the principal known variables (duration of enzymatic treatment, enzyme concentration and type of antibody). The results are discussed with reference to those previously obtained by optical velocimetry.

Keywords: Laser-Doppler Electrophoresis, Surface Charge, Erythrocytes

DOI: 10.3233/CH-1990-10111

Journal: Clinical Hemorheology and Microcirculation, vol. 10, no. 1, pp. 103-111, 1990