Expression of adhesion molecules and collagen on rat chondrocyte seeded into alginate and hyaluronate based 3D biosystems. Influence of mechanical stresses
Issue title: 3rd International Symposium on Mechanobiology of Cartilage and Chondrocyte. Brussels, May 16–17, 2003
Affiliations: Mécanique et Ingénierie Cellulaire et Tissulaire, UMR‐CNRS‐UHP‐INPL 7563 LEMTA et IFR 111, Faculté de médecine, 54505 Vandoeuvre‐lès‐Nancy, France | Laboratoire de Chimie Physique Macromoléculaire, UMR CNRS 7568 et IFR 111, Groupe ENSIC, 54000 Nancy, France | Physiopathologie et Pharmacologie Articulaires, UMR CNRS UHP 7561 et IFR 111, Faculté de médecine, 54505 Vandoeuvre‐lès‐Nancy, France
Note: [] Corresponding author: Céline Huselstein, Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA UMR CNRS 7563, Faculté de Médecine ‐ B.P. 184, 54505 Vandoeuvre‐lès‐Nancy Cedex, France. Tel.: +33 (0) 3 83 68 34 52; Fax: (33) 03 83 68 34 59; E‐mail: Celine.Huselstein@medecine.uhp‐nancy.fr.
Abstract: Chondrocytes use mechanical signals, via interactions with their environment, to synthesize an extracellular matrix capable to withstanding high loads. Most chondrocyte–matrix interactions are mediated via transmembrane receptors such as integrins or non‐integrins receptors (i.e. annexin V and CD44). The aim of this study was to analyze, by flow cytometry, the adhesion molecules (α5/β1 integrins and CD44) on rat chondrocytes seeded into 3D biosystem made of alginate and hyaluronate. These biosystems were submitted to mechanical stress by knocking the biosystems between them for 48 hours. The expression of type I and type II collagen was also evaluated. The results of the current study showed that mechanical stress induced an increase of type II collagen production and weak variations of α5/β1 receptors expression no matter what biosystems. Moreover, our results indicated that hyaluronan receptor CD44 expression depends on extracellular matrix modifications. Thus, these receptors were activated by signals resulted from cell environment variations (HA addition and modifications owing to mechanical stress). It suggested that this kind of receptor play a crucial role in extracellular matrix homeostasis. Finally, on day 24, no dedifferentiation of chondrocytes was noted either in biosystems or under mechanical stress. For all biosystems, the neosynthesized matrix contained an important level of collagen, which was type II, whatever biosystems. In conclusion, it appeared that the cells, under mechanical stress, maintained their phenotype. In addition, it seems that, on rat chondrocytes, α5/β1 integrins did not act as the main mechanoreceptor (as described for human chondrocytes). In return, hyaluronan receptor CD44 seems to be in relation with matrix composition.
Journal: Biorheology, vol. 41, no. 3-4, pp. 423-431, 2004
