Leucocyte removal prior to study of erythrocyte deformability

Issue title: New Methods in Biorheology. Proceedings of the International Symposium held in Nancy, France, 17–18 August 1983. A Satellite Symposium of the Fifth International Congress of Biorheology

Guest editors: J.F. Stoltz and E. Puchelle

Article type: Research Article

Authors: Stuart, J.^{; *} | Kenny, M.W. | Meakin, M. | Lucas, G.S. | Caldwell, N.M.

Affiliations: Department of Haematology, The Medical School, University of Birmingham, Birmingham B15 2TJ, UK

Correspondence: [*] Correspondence to Professor J. Stuart

Abstract: Posit.ive-pressure and initial-flow-rate (Hémorhéomètre) methods for the study of erythrocyte filtration through 5 μm diameter pores are highly sensitive to the presence of contaminating leucocytes in the erythrocyte test suspension. A pre-filtration step, in which heparinised or EDTA-anticoagulated whole-blood was passed through a column of Imugard IG500 cotton wool, was therefore developed. This procedure removed contaminating platelets and leucocytes, but not erythrocyte sub-populations, and is likely to improve the sensitivity and specificity of erythrocyte filtration techniques.

Keywords: Rheology, Leukocytes, Anticoagulants, Erythrocyte deformability

DOI: 10.3233/BIR-1984-23S150

Journal: Biorheology, vol. 23, no. s1, pp. 283-285, 1984