Fluorescence polarization applied to cellular microrheology

Article type: Review Article

Authors: Stoltz, J.F. | Donner, M.

Affiliations: INSERM - U.284, Plateau de Brabois, 54511 Vandoeuvre Les Nancy Cédex France

Abstract: The fluorescence polarization of probe molecules gives information on the “fluidity” of probe environment. Although the data cannot be related with absolute values of microviscosity, the method is largely used for probing the “fluidity” of lipid regions of biological membranes. Therefore, fluorescence polarization is of interest in clinical research, for membrane alterations are associated with either pathological processes of red cells, platelets, leukocytes or important cell functions.

Keywords: fluorescence polarization, probes, cell biology, lipid fluidity red cells, platelets, leukocytes, clinical applications

DOI: 10.3233/BIR-1985-22307

Journal: Biorheology, vol. 22, no. 3, pp. 227-247, 1985