Rheological behaviors of bovine blood forming artificial rouleaux

Issue title: Memorial Issue in Honor of Bun’ichi Tamamushi

Guest editors: Syoten Oka and Eiichi Fukada

Article type: Research Article

Authors: Kaibara, Makoto

Affiliations: The Institute of Physical and Chemical Research, Wako-shi, Saitama 351, Japan

Note: [] Accepted by: Editor S. Oka

Abstract: A purpose of the present study is to make an artificial rouleau of bovine red blood cells which is not capable of rouleau formation under physiological condition. Rheological behaviors of bovine blood forming artificial rouleaux were examined. The modification of cell surface by enzyme trypsin induced rouleau formation, whereas the modification of cell surface by neuraminidase did not cause any aggregate formation. The drastic elevation of the fibrinogen content in bovine red blood cells suspension also brought about the formation of rouleau. The value of dynamic rigidity modulus G′ of bovine red blood cells in saline solution containing high concentration of fibrinogen is somewhat smaller than that of trypsin treated bovine red blood cells in plasma. The value of G′ of trypsin treated bovine red blood cells in plasma first increased to a maximum value and then decreased with the time. It is supposed that the removal of macromolecules from the cell surface facilitates the mutual approach of cells and causes the formation of rouleau which seems to be the same as that of human and horse bloods.

Keywords: bovine blood, artificial rouleau, viscoelasticity, viscosity

DOI: 10.3233/BIR-1983-20515

Journal: Biorheology, vol. 20, no. 5, pp. 583-592, 1983