Measurement of the erythrocyte orientation in a flow by spin labeling

Subtitle: I/ Comparison between experimental and numerically simulated E.P.R. Spectra

Article type: Research Article

Authors: Bitbol, M. | Leterrier, F.

Affiliations: Division de Biophysique du C.R.S.S.A., 92141 Clamart

Note: [] Accepted by: Editor D. Quemada

Abstract: When a suspension of erythrocytes labeled in their membrane with a fatty acid paramagnetic molecule is allowed to flow in a flat quartz sample cell, the recorded electron paramagnetic spectra change as a function of the orientation of the cell in the magnetic field. This indicates that the red cells are themselves oriented in the flow. Such spectral variations have been reproduced by a numerical simulation procedure, which allowed us to quantify the proportion of oriented red blood cells by measuring the amplitude of sone characteristic lines on the experimental spectra. Orientation rates were then measured as a function of various rheological parameters, such as shear rate, hematocrit and viscosity of the suspending medium. The kinetics of the disorientation process was determined by stopping the flow.

Keywords: Spin label, Erythrocyte orientation

DOI: 10.3233/BIR-1982-19601

Journal: Biorheology, vol. 19, no. 6, pp. 669-680, 1982