Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Bozzo, Jordi | Tonda, Raúl | Hernández, María‐Rosa | Alemany, Mònica | Galán, Ana‐María | Ordinas, Antonio | Escolar, Ginés
Affiliations: Department of Hemotherapy and Hemostasis, Hospital Clinic, Barcelona, Spain
Note: [] Address for correspondence: Dr. Jordi Bozzo, Hospital Clinic, Servei d'Hemoteràpia i Hemostàsia, Villarroel, 170, 08036 Barcelona, Catalonia, Spain. Tel.: +34 932 275 400, ext. 2034, 2307; Fax: +34 932 279369; E‐mail: escolar@medicina.ub.es.
Abstract: We investigated whether ghosts behaved similarly to intact erythrocytes to maintain regular primary hemostasis under flow conditions. To this end we performed perfusion experiments with whole blood in which erythrocytes were replaced by pink ghosts, and platelet interaction with the subendothelial surface of a damaged vessel was morphometrically evaluated. The same objective was sought by means of studies with a platelet function analyzer (PFA‐100TM instrument). Perfusions performed with control blood reconstituted with intact erythrocytes gave rise to 0.4±0.2% contact but not spread platelets, 10.8±3.4% adhering and spread platelets, 16.3±4.6% platelets in thrombi, with 27.5±7.4% of the surface covered. Even though the average diameter of the ghosts was smaller than that of intact erythrocytes (5.3 μm vs. 7.7 μm), the values obtained in perfusions performed with ghosts were similar to those of the erythrocyte controls. Studies performed with the PFA‐100TM analyzer were consistent with those observed in perfusion studies. The viscosity of control blood was compared with that of blood reconstituted with ghosts. At shear rates lower than 450 s−1, the viscosity of the ghost samples was higher than that of the controls, but the difference progressively decreased as shear rate increased up to 750 s−1 (3.61±0.15 and 3.71±0.17 cP, respectively). In conclusion, the results of our study showed that ghosts behaved similarly to intact erythrocytes in maintaining a normal platelet interaction with digested subendothelium, under conditions of moderate shear rate and constant hematocrit (40%). The rheological activity of ghosts, bodies that are metabolically less active, was sufficient for them to satisfactorily act as substitutes for intact erythrocytes in our system.
Keywords: Erythrocytes, ghosts, platelets, perfusion
Journal: Biorheology, vol. 38, no. 5-6, pp. 429-437, 2001
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
sales@iospress.com
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
info@iospress.nl
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office info@iospress.nl
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
china@iospress.cn
For editorial issues, like the status of your submitted paper or proposals, write to editorial@iospress.nl
如果您在出版方面需要帮助或有任何建, 件至: editorial@iospress.nl