Journal of Berry Research - Volume 11, issue 4 - Journals

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Identification of R2R3-MYB gene family reveal candidate genes for anthocyanin biosynthesis in Lonicera caerulea fruit based on RNA-seq data

Authors: Gang, Huixin | Zhang, Qian | Chen, Jing | Qin, Dong | Huo, Junwei

Article Type: Research Article

Abstract: BACKGROUND: R2R3-MYB transcription factor (TF) family plays important roles in various biological processes in many plants, especially in the regulation of plant flavonoid accumulation. The fruit of Lonicera caerulea contains abundant anthocyanin. OBJECTIVE: The R2R3-MYB TF family was systematically analyzed according to the RNA-seq data, and the R2R3-MYB candidate genes that were involved in anthocyanin biosynthesis in the fruit of Lonicera caerulea were screened. METHODS: The R2R3-MYB TFs in Lonicera caerulea were identified, and the physical and chemical properties, protein conserved sequence alignment and motifs of each R2R3-MYB TFs were analyzed using …bioinformatics methods. The expression levels of these genes and anthocyanin levels in different tissues and different developmental stages of fruit were determined by RT-qPCR and pH shift method. RESULTS: A total of 59 genes encoding R2R3-MYB TFs in Lonicera caerulea were identified and clustered into 20 subgroups (C1 to C20) based on the relationship to AtR2R3-MYBs. Expression profiles showed that the expression of CL6086 and CL552 in fruit were higher than other tissues, and upregulated in the veraison fruit compared to the green ripe fruit. As the expression of the two genes was concurrent with the anthocyanin content, and showed high correlation with anthocyanin biosynthetic structural genes, they were considered as closely related to anthocyanin biosynthesis in the fruit. CONCLUSION: The results provide a systematic analysis of LcR2R3-MYBs, and the foundation for further molecular mechanisms research of anthocyanin biosynthesis regulated by R2R3-MYB in the fruit of Lonicera caerulea . Show more

Keywords: Lonicera caerulea, R2R3-MYB, anthocyanin, fruit, expression pattern

DOI: 10.3233/JBR-210008

Citation: Journal of Berry Research, vol. 11, no. 4, pp. 669-687, 2021

Price: EUR 27.50

Polyphenol contents, antioxidant activities, and anti-cancer cell proliferation properties at each stage of fruit development in intersectional hybrids between highbush blueberry and shashanbo ( Va…

Authors: Toyama, Yuki | Toshima, Saki | Hirano, Tomonari | Yamasaki, Masao | Kunitake, Hisato

Article Type: Research Article

Abstract: BACKGROUND: In blueberries, the total polyphenol contents, total anthocyanin contents, and antioxidant activities of fruits in blueberries and their relatives are becoming important targets for breeders. Recently, intersectional hybrids between Japanese wild species Shashanbo and highbush blueberry were produced using polyploid breeding for the first time. However, the polyphenol contents, antioxidant activities, and anti-cancer cell proliferation properties of the fruits of these intersectional hybrids have not been studied sufficiently. OBJECTIVE: The objectives of this study were to determine the anthocyanin contents, polyphenol contents, and antioxidant activities at each stage of fruit development in intersectional hybrids, and to examine …the effects of the fruit extracts on anti-cancer cell proliferation properties using human promyelocytic leukemia (HL-60) cells. METHODS: Freeze-dried fruit samples of three intersectional hybrids and their parents at five developmental stages were used to evaluate their polyphenol contents, anthocyanin contents, and levels of two types of antioxidant activities (DPPH and ORAC). The anti-cancer cell proliferation properties of each extract were also evaluated according to the survival rate of HL-60 cells. RESULTS: The total polyphenol contents in fruits of the intersectional hybrids gradually decreased as the fruits grew, but the contents increased slightly at the mature stage. Meanwhile, the total anthocyanin contents increased strongly to the mature stage. In addition, the fruit extract of intersectional hybrid JM1 was found to be more effective for inhibiting the growth of HL-60 human leukemia cells in vitro . Total polyphenol content correlated highly with antioxidant activities and anti-cancer cell proliferation properties. CONCLUSIONS: We clarified that the intersectional hybrids had produced have abundant bioactive compounds, high levels of antioxidant activities, and anti-cancer cell proliferation properties. The results proved the effectiveness of the use of Japanese wild Vaccinium species for the strategic breeding of blueberries with high levels of bioactive compounds. Show more

Keywords: Intersectional hybrids, anthocyanins, polyphenols, chlorogenic acid, anti-cancer cell proliferation property, chlorogenic acid

DOI: 10.3233/JBR-210713

Citation: Journal of Berry Research, vol. 11, no. 4, pp. 689-704, 2021

Price: EUR 27.50

ABA accelerates blackberry ( Rubus spp.) fruit ripening by positively affecting ripening-related gene expression and metabolite profiles

Authors: Zhang, Chunhong | Wu, Yaqiong | Xiong, Zhenghao | Li, Weilin | Wu, Wenlong

Article Type: Research Article

Abstract: BACKGROUND: The softness of blackberry fruits limits their postharvest shelf-life and commercial use, and abscisic acid (ABA) is considered one of the key hormones involved in fruit ripening. OBJECTIVE: This study aimed to explore the underlying physiological and molecular actions of ABA on blackberry fruit ripening and softening. METHODS: Various physiological indices of and plant hormone levels in treated and untreated blackberry fruits were determined simultaneously. The differentially expressed genes (DEGs) were analyzed by RNA-sequencing, and their expression profiles were detected. The ripening mechanism was elucidated by UHPLC-MS using two groups of fruits at 28 d. …RESULTS: After 25 d, the ABA concentration and polygalacturonase (PG) and beta-1,4-endoglucanase (EG) activities in ABA-treated fruits were significantly higher than those in untreated fruits. Large differences in the expression profiles were detected at 28 d. The expression of DEGs related to cell wall softening and ABA synthesis was largely triggered after 25 or 28 d. Sixty-nine differentially accumulated metabolites were ultimately annotated as related to fruit ripening. CONCLUSIONS: ABA stimulates blackberry fruit ripening by promoting cell wall enzyme activities, the expression of various ripening-related genes and metabolite accumulation. Show more

Keywords: Blackberry (Rubus spp.), ABA, transcriptome, fruit ripening and softening

DOI: 10.3233/JBR-210725

Citation: Journal of Berry Research, vol. 11, no. 4, pp. 705-720, 2021

Price: EUR 27.50

Bibliometric analysis of strawberry ( Fragaria × ananassa Duch.) research publications from horticulture category based on Web of Science

Authors: Yuan, Bao-Zhong | Sun, Jie

Article Type: Research Article

Abstract: BACKGROUND: The strawberry (Fragaria × annanasa Duch.) is one of the most significant cash horticultural crops in the world. Fresh strawberry fruits contain dietary fiber, vitamin C, 30-08-2021-carotene, folic acid and other nutrients required for human health and nutrition, and they have an appetizing aroma and flavour, as well as various phytonutrients that may be potentially beneficial to human health and could be used against chronic diseases including cancer and cardiovascular disorders. OBJECTIVE: The current study aimed to identify and analyze the 2,640 article and review type papers of strawberry research from horticulture category based on …the Web of Science. METHODS: The Web of Science database was searched to extract data. Bibliometric data, including citation count, were analyzed together with the words by bibliometric science mapping and visualization tools. RESULTS: Papers mainly written in English (2,426, 91.894 %), were from 6,807 authors, 77 countries/territories, 1,504 organizations and published in 57 Journals and book series. Top 5 core journals ranked as Hortscience (360, 13.636 %), Scientia Horticulturae (322, 12.197 %), Journal of the American Society for Horticultural Science (248, 9.394 %), Postharvest Biology and Technology (236, 8.939 %) and Journal of Horticultural Science Biotechnology (134, 5.076 %), that these journals each published more than 134 papers. Top 5 countries and regions were USA, Peoples R China, Japan, South Korea and Brazil. Top 5 organizations were Univ Florida, Univ Calif Davis, USDA ARS, ARS and Michigan State Univ. Top 5 authors are Hancock James F. (Hancock JF), Shaw DV, Chandler Craig K. (Chandler CK), Whitaker Vance M, Choi Jong Myung, each published more than 28 papers. All keywords of the strawberry research from horticulture Category were separated eight clusters for different research topics. CONCLUSIONS: Visualizations offer exploratory information on the current state in a scientific field or discipline as well as indicate possible developments in the future. The review could provide a valuable guide for designing future studies. This work is useful for student identifying graduate schools and researchers selecting journals. Show more

Keywords: Bibliometric analysis, Strawberry (Fragaria×ananassa Duch.), horticulture category, VOSviewer, web of science

DOI: 10.3233/JBR-210741

Citation: Journal of Berry Research, vol. 11, no. 4, pp. 721-738, 2021

Price: EUR 27.50

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Journal of Berry Research - Volume 11, issue 4

Identification of R2R3-MYB gene family reveal candidate genes for anthocyanin biosynthesis in Lonicera caerulea fruit based on RNA-seq data

Polyphenol contents, antioxidant activities, and anti-cancer cell proliferation properties at each stage of fruit development in intersectional hybrids between highbush blueberry and shashanbo ( Va…

ABA accelerates blackberry ( Rubus spp.) fruit ripening by positively affecting ripening-related gene expression and metabolite profiles

Bibliometric analysis of strawberry ( Fragaria × ananassa Duch.) research publications from horticulture category based on Web of Science

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