Journal of Berry Research - Volume 10, issue 2

Authors: Luo, Ting | Chen, Sirui | Zhang, Haihua | Jia, Shijie | Wang, Jinling

Article Type: Research Article

Abstract: BACKGROUND: The scientific interest regarding raspberry leaf composition and potential benefits grow. Still, little is known about raspberry leaves. OBJECTIVE: Extracts from nine raspberry leaf species and Autumn Bliss raspberry leaf tea (LT) were evaluated for their phenolic characteristics along with potential biological activities. METHODS: Phenolic constituents in leaf extracts from LT and nine raspberry species, including European red raspberry (ERR), Australian red raspberry (ARR), U.S. Raspberry (USR), Autumn Bliss (AB), Heritage (H), Tulameen (T), Longyuan Qiufeng (LQ), Qiuping (Q), and Fengman Hong (FH) were evaluated by UPLC-MS. Regarding to the bioactive activities, antioxidant activities (DPPH …and ABTS assays), antidiabetic properties (α -amylase inhibition and α -glucosidase inhibition assays), antibacterial activities (growth inhibition zone measurement and a minimum inhibitory concentration (MIC)), and anti-inflammatory properties (inhibition of hyaluronidase activity and inhibition of albumin denaturation), were investigated. RESULTS: The results showed that ERR extract exhibited the highest levels of total phenolic (5.56±0.058 mg/g), the highest chlorogenic acid (597.03±10.90 mg/kg) and rutin level (2771.9±41.52 mg/kg). Autumn Bliss LT possessed the lowest total phenolics, and lowest DPPH scavenging activity. A high correlation between phenolic content and antioxidant activity was found in these extracts. CONCLUSIONS: High phytochemical contents and biological activities of these raspberry leaves, including antioxidant, antidiabetic, antibacterial, and anti-inflammatory effect were shown, which indicated their consumption would be beneficial to health. Show more

Keywords: Raspberry leaf, phytochemicals, antioxidant, antibacterial, antidiabetic, anti-inflammatory

DOI: 10.3233/JBR-190474

Citation: Journal of Berry Research, vol. 10, no. 2, pp. 295-309, 2020

Authors: Liao, Xiaoxi | Greenspan, Phillip | Srivastava, Anita | Pegg, Ronald B.

Article Type: Research Article

Abstract: BACKGROUND: Georgia’s small fruit sector is important to the state’s agricultural economy, and is seeking ways to promote their fruits in the health and wellness market. Based on the hot, humid, climatic conditions of the U.S. Southeast, three blackberry varieties, namely ‘Navaho’, ‘Ouachita’, and ‘Kiowa’, can grow, but a complete characterization of their antioxidant phenolic constituents has not been reported. OBJECTIVE: This study aims to investigate the phenolic compounds and antioxidant activities in U.S. Southeastern erect-type blackberry cultivars of commercial significance. METHODS: Phenolics from blackberries were isolated, separated, and elucidated both qualitatively and quantitatively by various …chromatographic means, including HPLC–ESI–MS and MALDI–TOF–MS. RESULTS: Delphinidin-3-O -glucoside, malvidin-3-O -glucoside, quercetin-3-O -glucoside malonate, and propelargonidin B-type dimer were present in the Southeastern blackberries, which have not been found in any major U.S. cultivars. MALDI–TOF–MS analysis revealed a distinctive ellagitannin profile among the cultivars, and identified 5 additional ellagitannin compounds that were not elucidated by HPLC–ESI–MS.The detection of the propelargonidin B-type dimer revealed that the flavan-3-ols of blackberries are not limited to (epi)catechin-based compounds. CONCLUSIONS: U.S. Southeastern erect-type blackberry cultivars grown in Georgia showed distinct phenolic profiles and antioxidant activities from those of Pacific Northwestern cultivars. Show more

Keywords: Blackberries, phenolics, anthocyanins, ellagitannins, ESI–MS, MALDI–TOF–MS

DOI: 10.3233/JBR-190475

Citation: Journal of Berry Research, vol. 10, no. 2, pp. 311-327, 2020