Cancer Biomarkers - Volume 19, issue 1

Authors: Cheng, Jingliang | Fu, Shangyi | Wei, Chunli | Tania, Mousumi | Khan, Md. Asaduzzaman | Imani, Saber | Zhou, Baixu | Chen, Hanchun | Xiao, Xiuli | Wu, Jingbo | Fu, Junjiang

Article Type: Research Article

Abstract: BACKGROUND: PIK3CA gene encodes the p110 α catalytic subunit of the oncoprotein phosphatidylinositol 3-kinase (PI3 K) which regulates many biological processes such as cell proliferation, differentiation, migration and survival through the activation of various signaling pathways. OBJECTIVE: In this study, we have investigated the possible somatic mutations in PIK3CA gene in invasive ductal breast carcinomas of Chinese women from Western China. METHODS: Genomic DNA was extracted from the formalin-fixed paraffin-embedded (FFPE) tissue samples. The hotspot mutations in PIK3CA gene of exon 9 and exon 20 were studied by pyrosequencing. …RESULTS: The sequencing identified two hotspot mutations in exon 20 of one cancer samples at p. H1047L (c. 3140A > T) and eight cancer sample at p. H1047R (c. 3140A > G). No mutation in exon 9 of PIK3CA gene was found in these breast cancer tissue samples. PIK3CA mutations showed surprising clinicopathological features in breast cancer patients, as incidence of lymph node invasiveness is increased in the patients with PIK3CA mutation. In addition, all the patients showed tumor size bigger than 3 cm in diameter. It is important that for early detection and early treatment for BC in developing countries or areas like Western China, and for people to provide popularization education using scientific knowledge in cancer fields. CONCLUSIONS: This study identified PIK3CA mutations in breast carcinoma patients of Western China that will enable a more rapid molecular diagnosis, and provide a stronger rationale evidence for development of precision therapeutic approaches as well as promising therapeutic targets for breast cancer treatment or patient management. Show more

Keywords: Breast cancer, phosphatidylinositol 3-kinase, PIK3CA gene, DNA mutation, pyrosequencing, Western China

DOI: 10.3233/CBM-160380

Citation: Cancer Biomarkers, vol. 19, no. 1, pp. 85-92, 2017

Authors: Yuan, Qiuyue | Sun, Tingge | Ye, Feng | Kong, Weisheng | Jin, Haofan

Article Type: Research Article

Abstract: OBJECTIVE: The aim of this study was to establish the relationship between miR-124-3p and Aurora A kinase (AURKA ) in bladder cancer (BC). METHODS: The expressions of miR-124-3p and AURKA in BC tissues and cell lines were detected using RT-PCR and western blot. BC cells were transfected with miR-124-3p mimics and AURKA siRNA. After this cell proliferation, migration, cell cycle and apoptosis were measured using CCK-8, colony formation assay, wound healing assay and cytometry tests. The relationship between miR-124-3p and AURKA was confirmed with luciferase reporter assay. Mice xenograft models were constructed to …examine the effects of AURKA on BC in vivo . RESULTS: MiR-124-3p expression was significantly down-regulated in BC tissues and cell lines, while AURKA was significantly up-regulated compared to normal samples. MiR-124-3p targeted AURKA and decreased its expression. Transfection of miR-124-3p mimics and AURKA siRNA was shown to down-regulate BC cell proliferation and migration as well as induce cell apoptosis. As suggested by xenograft models, the inhibition of AURKA can effectively suppress tumor growth. CONCLUSION: MiR-124-3p has significant impact on proliferation, migration and apoptosis of BC cells by targeting AURKA . Show more

Keywords: MicroRNA-124-3p, AURKA, bladder cancer

DOI: 10.3233/CBM-160427

Citation: Cancer Biomarkers, vol. 19, no. 1, pp. 93-101, 2017

Authors: Hong, Tingting | Shen, Di | Chen, Xiaoping | Wu, Xiaohong | Hua, Dong

Article Type: Research Article

Abstract: BACKGROUNDS: Cancer progression has been associated with host hemostasis system. Whether preoperative plasma hemostasis factors can predict survival in colorectal cancer is quite intriguing. METHODS: We conducted a prospective cohort study to validate the prognostic significance of three hemostasis parameters - fibrinogen, fibrin degradation products (FDPs) and D-dimer - in non-metastatic colorectal cancer patients treated with curative resection. RESULTS: All three parameters were positively correlated with C reactive protein (CRP) levels and Glasgow Prognostic scores (GPS). In univariate cox hazards regression model, as continuous variables, both fibrinogen (HR: 1.07, 95%CI: 1.01-1.13) and FDPs …(HR: 1.17, 95%CI: 1.05-1.31) were prognostic, while D-dimer levels were not. Patients with hyperfibrinogenemia had a 2.12-fold increased mortality risk compared with patients without hyperfibrinogenemia. Patients with positive FDPs had a 3.68-fold increased mortality risk compared with patients with negative FDPs. In multivariate models, hyperfibrinogenemia was prognostic (HR: 3.39, 95%CI: 1.34-8.67) in patients with normal GPS scores. CONCLUSIONS: Preoperative fibrinogen levels appeared as an independent mortality risk factor in non-metastatic colorectal cancer patients with normal GPS scores. Fibrinogen could be a reliable marker to identify high risk patients for those without systematic inflammation responses. Show more

Keywords: Colorectal neoplasms, survival, fibrinogen, fibrin/fibrinogen degradation products

DOI: 10.3233/CBM-160510

Citation: Cancer Biomarkers, vol. 19, no. 1, pp. 103-111, 2017

Authors: Xu, Ying-Jie | Qian, Chen | He, Jun | Cheng, Da-Qing | Jiang, Liu-Xia

Article Type: Case Report

Abstract: Here one concurrent case of a patient with both thyroid and breast cancers was discussed with a comprehensive literature review. On March 21, 2016, a 42-year-old female patient was admitted into our hospital because of right neck mass and left breast mass for 1 week during a routine health examination. On March 22, the combined procedures of complete resection of right thyroid gland plus isthmus with dissection of lymph nodes in right VI region plus left breast regional lumpectomy were performed under endotracheal anesthesia. Final diagnosis: Mammary cancer (right thyroid gland), PT1aN1aM0 (stage I); ductal breast cancer (left breast), PT1NxM0 …(re-operation within two weeks). For either disease, a practicing clinician should stay on a high alert for individual and familial endocrine diseases. And breast molybdenum target imaging and type B ultrasound should be performed regularly for screening. It is always prudent to catch and treat diseases as early as possible. The conclusion is as follows. Show more

Keywords: Thyroid cancer, breast cancer, receptor regulations, correlations, gene level

DOI: 10.3233/CBM-160505

Citation: Cancer Biomarkers, vol. 19, no. 1, pp. 113-117, 2017