Affiliations: [a] Cancer Cente, The First Hospital of Jilin University, Changchun, Jilin 130021, China | [b] BASO Cell Science and Technology Co., Ltd., Zhuhai, Guangdong 519015, China
Correspondence:
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Corresponding author: Haofan Jin, Cancer Center, The First Hospital of Jilin University, No.71 Xinmin Street, Changchun, Jilin 130021, China. Tel./Fax: +86 431 88783135; E-mail: gzzhaozhjian@126.com
Abstract: OBJECTIVE: The aim of this study was to establish the relationship
between miR-124-3p and Aurora A kinase (AURKA) in bladder cancer (BC).
METHODS: The expressions of miR-124-3p and AURKA in BC tissues and cell
lines were detected using RT-PCR and western blot. BC cells were transfected
with miR-124-3p mimics and AURKA siRNA. After this cell proliferation, migration,
cell cycle and apoptosis were measured using CCK-8, colony formation assay,
wound healing assay and cytometry tests. The relationship between miR-124-3p
and AURKA was confirmed with luciferase reporter assay. Mice xenograft models
were constructed to examine the effects of AURKA on BC in vivo.
RESULTS: MiR-124-3p expression was significantly down-regulated in
BC tissues and cell lines, while AURKA was significantly up-regulated compared to
normal samples. MiR-124-3p targeted AURKA and decreased its expression.
Transfection of miR-124-3p mimics and AURKA siRNA was shown to down-regulate BC
cell proliferation and migration as well as induce cell apoptosis. As
suggested by xenograft models, the inhibition of AURKA can effectively suppress
tumor growth.
CONCLUSION: MiR-124-3p has significant impact on proliferation,
migration and apoptosis of BC cells by targeting AURKA.
