Bio-Medical Materials and Engineering - Volume 19, issue 4-5

Authors: Petitpain, Nadine | Gambier, Nicolas | Wahl, Denis | Chary-Valckenaere, Isabelle | Loeuille, Damien | Gillet, Pierre

Article Type: Research Article

Abstract: Background: Systemic inflammation such as rheumatoid arthritis (RA) and Crohn's disease (CD) may be responsible for vascular comorbidity. TNF-α blockade was expected to lower these comorbidities but several cases of arterial and venous thromboembolic events (TE) have been reported. Objectives: The aim of this work was to study retrospectively the main characteristics of spontaneously notified TNF-α blockers related TE over a 7-year period. Methods: TE related to infliximab, etanercept and adalimumab spontaneously notified to the French adverse drug reporting system database between January 2000 and December 2006 were analyzed. Separate analysis of arterial TE and venous TE was …performed. Risk factors for each category of TE were assessed with consensual criteria. Results: 85 TE were analyzed, representing 4.5% of all the spontaneously notified adverse reactions of the 3 TNF-α blockers in the database. 42 were arterial events and 43 were venous events. The incidence was not significantly different between the 3 TNF-α blockers. Mean delay of TE onset after treatment initiation was 10.6 months. It was significantly shorter for etanercept (6.1 months, p=0.001) especially for venous TE (2.4 months). 16 among the 42 patients with arterial TE had 2 or more risk factors whereas 39 among the 43 patients with venous TE had no RF or only one. Most of patients (79/85) received concomitant systemic corticosteroids and/or methotrexate and/or COX-2 selective inhibitors. 23 patients had been investigated for autoimmunity, 13 had antinuclear and/or antiphospholipid antibodies. Main limitations of this study were underreporting and heterogeneous report contents. Conclusion: Despite its limitations, this study suggests that venous TE could be favoured by TNF-α blockers therapy since they occurred in patients with no or few risk factors for venous thrombosis. However, this needs to be more evaluated by controlled studies. Show more

Keywords: Arthritis, rheumatoid, complications, drug therapy, physiopathology, cardiovascular diseases, etiology, mortality, tumor necrosis factor-alpha, antagonists and inhibitors

DOI: 10.3233/BME-2009-0600

Citation: Bio-Medical Materials and Engineering, vol. 19, no. 4-5, pp. 355-364, 2009

Authors: Auxenfans, Céline | Thépot, Amélie | Justin, Virginie | Hautefeuille, Agnès | Shahabeddin, Lili | Damour, Odile | Hainaut, Pierre

Article Type: Research Article

Abstract: Large-scale culture of primary keratinocytes allows the production of large epidermal sheet surfaces for the treatment of extensive skin burns. This method is dependent upon the capacity to establish cultures of proliferating keratinocytes in conditions compatible with their clonal expansion while maintaining their capacity to differentiate into the typical squamous pattern of human epidermis. Feeder layers are critical in this process because the fibroblasts that compose this layer serve as a source of adhesion, growth and differentiation factors. In this report, we have characterise the expression patterns of p63 isoforms in primary keratinocytes cultured on two different feeder layer systems, …murine 3T3 and human fibroblasts. We show that with the latter, keratinocytes express a higher ratio of ΔN to TAp63 isoform, in relation with higher clonogenic potential. These results indicate that human fibroblasts represent an adequate feeder layer system to support the culture of primary human keratinocytes. Show more

Keywords: Feeder layer, 3T3 fibroblasts, human fibroblasts, keratinocytes culture, ΔN/TAp63

DOI: 10.3233/BME-2009-0601

Citation: Bio-Medical Materials and Engineering, vol. 19, no. 4-5, pp. 365-372, 2009

Authors: Caunday, Olivia | Bensoussan, Danièle | Decot, Véronique | Bordigoni, Pierre | Stoltz, Jean François

Article Type: Review Article

Abstract: In 1997, the Joint Accreditation Committee ISCT & EBMT (JACIE) was created. The following year, it approved the first edition of standards for haemopoietic progenitor cell collection, processing and transplantation. The purpose of the standards is to ensure a minimal level of quality, alertness and homogeneity in the implementation of autologous and allogeneic haemopoietic stem cell transplantation (HSCT) programme in onco-hematology. The acquisition of accreditation is based upon the system of examination by trained medical professionals according to countries endorsements with the national regulation obligations applicable to HSCT. In 2008, the fourth edition has been launched. The range …of application of the standards comprises both donors and recipients, and all phases of collection, processing, storage and administration of haemopoietic progenitor cells. Among the accredited processing facilities, a few have been integrated JACIE standards into their existing management quality system which is inspected by national health authority. In France, the comparison between JACIE standards and the good manufacturing practices of cellular therapy product reveals some common points and some differences to apply. Show more

DOI: 10.3233/BME-2009-0602

Citation: Bio-Medical Materials and Engineering, vol. 19, no. 4-5, pp. 373-379, 2009