Antispasmodic effects of black chokeberry (Aronia melanocarpa (Michx.) Elliott) extracts and juice and their potential use in gastrointestinal disorders
Affiliations: [a] Department of Pharmacy, Faculty of Medicine, University of Niš, Niš, Serbia
| [b] Department of Physiology, Faculty of Medicine, University of Niš, Niš, Serbia
| [c]
Institute for Medicinal Plants Research “Josif Pančić“, Belgrade, Serbia
| [d] Faculty of Medicine, University of Niš, Niš, Serbia
Correspondence:
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Corresponding author: Dušanka Kitić, Department of Pharmacy, Faculty of Medicine, University of Niš, Avenue Zoran Djindjić 81, 18 000 Nis, Serbia. Tel.: +381 18 42 26 712; Fax: +381 18 42 38 770; E-mail: dusankakitic@gmail.com; dusanka.kitic@medfak.ni.ac.rs.
Abstract: BACKGROUND:Phytotherapy has an important role in the functional gastrointestinal and motility disorder prevention and treatment. OBJECTIVE:The aims of our study were to analyze the chemical composition and the antispasmodic activity of the dry black chokeberry ethanolic extract, waste extract and the juice in the isolated rat ileum. METHODS:The anthocyanins and flavonoids quantification was carried out by the spectrophotometric method using the HPLC instrument. The study examined the influence of the chokeberry preparations and cyanidin-3-O-galactoside, the main anthocyanin compound, on the spontaneous, acetylcholine and KCl-induced ileum smooth muscle contraction, as well as on the contractions induced by histamine, CaCl2 and BaCl2 and contractions in the presence of nitric oxide synthase inhibitor (L-NAME). RESULTS:In all the examined preparations, the most abundant compounds were anthocyanins, especially cyanidin heterosides. The highest content of polyphenols and anthocyanins was found in the chokeberry waste extract. Among the chokeberry preparations, the ethanolic extract had the highest flavonoid concentration. The chokeberry preparations and cyanidin-3-O-galactoside in a concentration dependent manner significantly relaxed the rat ileum spontaneous and induced contractions. The results demonstrated that the nitric-oxide pathway was not involved in the relaxant activity of the chokeberry extracts and juice. CONCLUSIONS:The study demonstrated that the chokeberry preparations were able to relax contractions in the isolated rat ileum.
