Bacteriophage cloning and Escherichia coli expression of a human IgM Fab

Article type: Research Article

Authors: Hay, Beverly N.^a | Sorge, Joseph A.^b | Shopes, Bob^a;

Affiliations: [a] Stratacyte Corporation, La Jolla, CA, USA | [b] Stratagene Cloning Systems, La Jolla, CA, USA

Note: [] Address reprint requests to Dr. Bob Shopes, Stratacyte Corporation, 11099 North Torrey Pines Road, Suite 400, La Jolla, CA 92037, USA.

Abstract: We have combined the molecular biology methods of the polymerase chain reaction and recombinant DNA cloning in bacteriophage fambda to express a human IgM Fab in Escherichia coli using genes derived from an Epstein-Barr vzrus transformed cell line. This method comprises three cDNA amplifications and a single clonzng step, culminating In the stable overexpression of mammalian heterodimeric recombinant protein in a prokaryotic host.

Keywords: antibody, immunoglobulin, gene expression, polymerase chain reaction

DOI: 10.3233/HAB-1992-3204

Journal: Human Antibodies, vol. 3, no. 2, pp. 81-85, 1992