Affiliations: [a] Division of Rheumatology, Allergy and Clinical Immunology, Department of Internal Medicine, University of California at Davis, School of Medicine, Davis, CA, USA | [b] Institute of Bio-Active Science, Nippon Zoki Pharmaceutical Co., Ltd., Hyogo, Japan
Note: [] Address reprint requests to Dick L. Robbins, M.D., Division of Rheumatology, Allergy and Clinical Immunology, University of California at Davis, TB 192, School of Medicine, Davis, CA 95616, USA.
Abstract: IgM rheumatoid factors (RF) are the predominant autoantibody found in rheumatoid arthritis. They are polyclonal, fix complement, and are directed against epitopes in the Fc portion of IgG. One hypothesis regarding the induction and persistence of RF production in rheumatoid arthritis is that the Fc of IgG is somehow altered, rendering it antigenic. In this study, to better understand the derivation and pathogenicity of RF in rheumatoid arthritis, monoclonal IgG (mIgG) constitutively secreting hybridomas were established by fusing rheumatoid synovial mononuclear cells (RSC) from patients with a mouse/human heteromyeloma cell line, F3B6. To clarify the primary structure of IgG Fc constant regions produced locally by RSC, we amplified the cDNA corresponding to the CH2 and CH3 domains of an IgGI-, IgG2-, and an IgG3-producing hybridoma derived from RSC. The amplified DNA segments were cloned in MI3 vectors and sequenced. Interestingly, very few differences in the nucleotide sequences were observed, and the deduced amino acid sequences were identical, except for the allotype, with those encoded by the human germline genes GEA and CL. Thus, the primary structure of the IgGI, IgG2, and IgG3 Fc regions produced by RSC were not altered when compared with those encoded by the unmutated human germline gene. These results suggest that factors other than altered IgG induce and sustain high avidity RF production in rheumatoid arthritis.
Keywords: rheumatoid arthritis, rheumatoid factors, human hybridomas
