Novel combination with maca improves sperm parameters in vitro of asthenozoospermic men

Article type: Research Article

Authors: Abdulzahra, Israa Saleem^{a; *} | Al-Dujaily, Saad Salih^b | Zabbon, Areej Abbas^a

Affiliations: [a] Department of Biology, College of Science, Mustanceryia University, Baghdad, Iraq | [b] Department of Physiology, High Institute of Infertility Diagnosis and Art, Al-Nahrain University, Baghdad, Iraq

Correspondence: [*] Corresponding author: Israa Saleem Abdulzahra, Department of Biology, College of Science, Mustanceryia University, Baghdad, Iraq. E-mail: saleem2975649@gmail.com, Israasaleem@uomustansiriyah.edu.iq.

Abstract: BACKGROUND: Infertility is an inability to conceive after a reasonable period of time (12 months) without the use of contraception or due to a person’s incapacity to procreate, whether independently or with a spouse. Problems with the production and maturation of sperm are the most common causes of male infertility additionally; the motility is the major functional character that determines the fertilizing ability of spermatozoa. Therefore the goal of this study is to get better certain sperm function parameters in vitro of asthenozoospermic patient. OBJECTIVE: The World Health Organization (WHO) and many studies considered the infertility as a disease and so many couples complaining from unsuccessful assisted reproductive technologies (ART) procedures to overcome their problem. The goal of this study is to improve certain sperm function feature in vitro of asthenozoospermic semen patients by using combination of motility inducing namely; Maca, L-carnitine and Pentoxifylline that enhance the medium to improve certain sperm characters that might be utilized for ART centers. METHODOLOGY: Semen aliquots were collected from ninety patients with asthenozoospermia who participated in present study, the volume of semen samples with normal ejaculate when was ranged between 1.4-6ml and can be measured by using a measure pipette or conical graduated tube; Inclusion criteria was Asthenozoospermia, oligozoospermia and teratozoospermia men, Infertile idiopathic men also, fertile normozoospermic men. While Exclusion criteria was Azoospermic men, Alcoholic, Patients under treatment with antibiotics and men with Varicocele.The samples split into two equal groups at random. Using Ham’s F12 medium, one portion served as the control group, and the other was the treatment group, which was mixing by combining the following ingredients, Maca powder extracts (Lepidium meyenii) (M) 1 mg/ml, 0.5 mg/ml of L-Carnitine (LC), and 10 mg/ml of Pentoxifylline (PTX). The data were analyzed using Statistical Package for Social Sciences (SPSS) version 23.0. The descriptive statistics including frequency, range, mean and standard error, Data from treated and control groups were expressed as mean ± SEM and to compare value between experimental and control groups using Students t-test. Layering approach is used to investigate sperm parameters before and after in vitro activation. RESULTS: The information showed a very large (p< 0.001) increase in active sperm motility grade A, percentage of progressive motility with significant increase in morphologically normal sperm (MNS) with decreased in DNA fragmentation index after activation by layering technique with novel combination medium compared to Hams F12 medium and before activation. CONCLUSION: The present work stated that novel combination medium (LC, maca and PXT) have potential effects to improve sperm characters in male infertility factors and suggested to be used for sperm preparation and activation in ART programs.

Keywords: L-carnitine, Pentoxifylline, maca powder, in vitro sperm preparation, DNA fragmentation index

DOI: 10.3233/HAB-240032

Journal: Human Antibodies, vol. 32, no. 4, pp. 221-227, 2024