Blood microbiota composition in Iranian pre-diabetic and type 2 diabetic patients¹

Article type: Correction

Authors: Ghaemi, Farahnaz^a | Fateh, Abolfazl^{b; c} | Sepahy, Abbas Akhavan^d | Zangeneh, Mehrangiz^e | Ghanei, Mostafa^f | Siadat, Seyed Davar^{b; c; g; *}

Affiliations: [a] Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran | [b] Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran | [c] Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran | [d] Department of Microbiology, Tehran North Branch, Islamic Azad University, Tehran, Iran | [e] Department of Infectious Diseases, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran | [f] Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran | [g] Endocrinology & Metabolism Research Center, Endocrinology & Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Correspondence: [*] Corresponding author: Seyed Davar Siadat, Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center, Pasteur Institute of Iran, No. 358, 12th Farvardin Ave, Jomhhoori St, Tehran 1316943551, Iran. E-mail: siadat@pasteur.ac.ir.

Note: [1] This article received a correction notice (Erratum) with the reference: 10.3233/HAB-229000, available at https://content.iospress.com/articles/human-antibodies/229000

Abstract: BACKGROUND: Type 2 diabetes as the most prevalent metabolic disorder, is one of the major causes of morbidity and mortality worldwide. Recent studies suggest that body microbiota may play a role in developing metabolic disorders including type 2 diabetes. The objective of the present study was to investigate the blood microbiota composition in Iranian pre-diabetic and type 2 diabetic patients compared to healthy individuals. METHODS: Blood samples were taken after 12-h fasting from 90 participants, 30 healthy individuals, 30 type 2 diabetes patients and 30 pre-diabetic participants. The buffy coat layer separated by centrifugation at 800 and DNA was extracted using a column-based method. Composition and load of blood microbiota was evaluated by real-time PCR method using genus specific 16S rRNA primers. RESULTS: The load of Akkermansia, and Faecalibacterium was higher in normal volunteers compared to pre-diabetic and type 2 diabetes group (p< 0.05).The load of Bifidobacterium was higher in normal volunteers compared to type 2 diabetes patients (p= 0.02).In contrast, the load of Lactobacillus and Escherichia coli was higher in pre-diabetics and type 2 diabetes patients compared to normal volunteers (p< 0.05).The load of Bacteroides fragilis was not statistically different between studied groups but it was higher in males compared to female group (p= 0.04). the load of other bacteria was not significantly different between male and female participants. CONCLUSION: There is difference between microbiota composition in white blood cells of pre-diabetic and type 2 diabetes patients compared to healthy people. Determination of blood microbiota pattern may have a role in diagnosis and preventive of type 2 diabetes in a certain population. For more clarification about correlation between blood microbiota and type 2 diabetes, larger studies with more participants in different ethnical populations is suggested.

Keywords: Microbiota, type 2 diabetes, pre-diabetes

DOI: 10.3233/HAB-210450

Journal: Human Antibodies, vol. 29, no. 4, pp. 243-248, 2021