Tomato endo beta-mannanase: A candidate of potential tomato allergen protein detected with human monoclonal antibody established from a patient suffered from Japanese cedar pollinosis
Affiliations: Graduate School of Natural Resources and Biology, Tamagawa University, Machida, Tokyo, Japan
Correspondence:
[*]
Corresponding author: Hiroshi Shinmoto, Graduate School of Natural Resources and Biology, Tamagawa University, 6-1-1 Tamagawa-gakuen, Machida, Tokyo 194-8610, Japan. Tel./Fax: +81 42 739 8267; E-mail: shnmt920@agr.tamagawa.ac.jp.
Abstract: Peripheral blood lymphocytes from a patient allergic to Japanese cedar pollens were transformed by Epstein-Barr virus infection. Some transformed B-lymphoblastoid cells (BLCs) secreted IgM class antibodies to cedar pollen extracts and tomato fruit extracts. One stable human-mouse hybridoma clone Y-22-3-3 secreting IgM class monoclonal antibody to tomato fruit extracts was established by cell fusion of BLCs with mouse myeloma cells. Western blot analysis of tomato extracts showed Y-22-3-3 monoclonal antibody recognized a tomato protein with a molecular weight of 40 kDa. The CBB-stained 40 kDa protein from antibody-affinity chromatography was analyzed by MALDI-TOF/TOF, and identified as tomato endo-beta-mannanase, which was previously reported as one of the potential candidates for tomato allergens.
Keywords: Japanese cedar pollen, tomato, allergy, monoclonal antibody, hybridoma
