Protein microarrays: A new tool for profiling antibody cross-reactivity

Article type: Research Article

Authors: Predki, Paul F.^{; *} | Mattoon, Dawn | Bangham, Rhonda | Schweitzer, Barry | Michaud, Gregory

Affiliations: Invitrogen Protein Microarray Center, 688 E Main St, Branford, CT 06405, USA

Correspondence: [*] Corresponding author. Tel.: +1 203 848 1101; Fax: +1 203 848 1155; E-mail: paul.predki@invitrogen.com.

Abstract: Antibody cross-reactivity can compromise interpretation of experiments and derail therapeutic antibody development. Standard techniques such as immunohistochemistry or Western analysis provide important but often inadequate approaches to assess antibody specificity. Protein microarrays are providing a new approach to rapidly characterize antibody cross-reactivity against 1,000s of proteins simultaneously. This review will focus on reported examples of antibody cross-reactivity, methods used to characterize them, and the recent development and use of protein microarrays for assessing antibody specificity.

Keywords: Antibody cross-reactivity, protein microarray, antibody specificity

DOI: 10.3233/HAB-2005-141-202

Journal: Human Antibodies, vol. 14, no. 1-2, pp. 7-15, 2005