Affiliations: Department of Periodontics, GDCRI, Bangalore, India
Note: [] Corresponding author: Department of Periodontics, Government
Dental College and Research Institute, Bangalore-560002, Karnataka, India.
Tel.: +91 9845081190; E-mail: periodontics_gdc@rediffmail.com
Abstract: Background: Plasma glutathione peroxidase (eGPx) is an important
selenium containing antioxidant in human defense against oxidative stress.
While crevicular fluid (GCF) eGPx levels and its association with periodontal
disease is well documented, there is no data on correlation of GCF and serum
eGPx levels in chronic periodontitis. Hence this study was undertaken to
further probe into the role of oxidative stress in periodontal diseases and
effect of nonsurgical periodontal therapy (NSPT) by correlating GCF and serum levels of eGPx. Materials and methods: Thirty subjects (16-Males and
14-Females; age: 30–38 years) participated in the study. The subjects were
divided, based on gingival index, probing pocket depth and clinical attachment
level into: Healthy (group-1, n=10), Gingivitis (group-2, n=10) and
Periodontitis (group-3, n=10). Chronic periodontitis patients after NSPT
constituted group 4. GCF and serum samples collected from each subject were
quantified for eGPx levels using Enzyme linked Immunosorbent Assay. Results: The mean eGPx concentrations increased from health (14.01 ng/μl
and 78.26 ng/ml) to gingivitis (22.86 ng/μl and 90.44 ng/ml) and then to
periodontitis (29.89 ng/μl and 103.43 ng/ml), in GCF and serum
respectively. After NSPT, there was statistically significant reduction in eGPx
concentration in GCF and serum (19.41 ng/μl and 85.21 ng/ml). Further, all
the GCF eGPx values showed a positive correlation to that of serum eGPx level. Conclusion: Thus, increased eGPx concentration in GCF can be considered
as an indicator of local increase in oxidative stress. While, increase in serum
eGPx levels indicates that periodontal disease can also lead to increased
oxidative stress at the systemic level.
