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Article type: Research Article
Authors: Navarro-Hernández, Rosa Elena | Oregon-Romero, Edith | Mercado, Mónica Vázquez-Del | Rangel-Villalobos, Héctor | Palafox-Sánchez, Claudia Azucena | Muñoz-Valle, José Francisco
Affiliations: Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara | Instituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara, Ocotlán, Jalisco, México
Note: [] Corresponding author: José Francisco Muñoz-Valle, PhD., Insurgentes 244-1, Colonia Lomas de Atemajac, Zapopan, Jalisco, C.P. 45178, México. Tel.: +52 33 10585309; E-mail: biologiamolecular@hotmail.com
Abstract: To investigate the association of sICAM-1 and sVCAM-1 with ICAM1 721G>A and VCAM1 1238G>C polymorphisms and rheumatoid arthritis (RA) clinical activity, sixty RA patients and 60 healthy non-related subjects (HS) matched for age and sex were recruited. Soluble adhesion molecules were determined by ELISA technique. Rheumatoid factor (RF), C reactive protein (CRP) and the erythrocyte sedimentation rate (ESR) were measured by routine methods. Disability and clinical activity was measured with Spanish-HAQ-DI and DAS28 scores, respectively. The ICAM1 and VCAM1 polymorphism were identified using the PCR-RFLP procedure. Inter-group comparison showed increased levels of sICAM-1 and sVCAM-1 in RA patients (284 and 481 ng/mL) versus HS (132 and 280 ng/mL); in the RA group, significant correlations between sVCAM-1 and RF (r = 0.402), ESR (r = 0.426), Spanish-HAQ-DI (r = 0.276), and DAS28 (r = 0.342) were found, whereas sICAM-1 only correlated with RF (r = 0.445). In RA patients, a significant association with the 721A allele of ICAM1 polymorphism (p = 0.04), was found. In addition, the allele impact (G/A+A/A) of this polymorphism was confirmed, (p = 0.038, OR = 2.3, C.I. 1.1–5.0). sVCAM-1 and sICAM-1 serum levels reflected the clinical status in RA, independently of the ICAM1 and VCAM1 polymorphism. However, the ICAM1 721A allele could be a genetic marker to RA susceptibility.
Keywords: Rheumatoid arthritis, polymorphism, soluble adhesion molecules, ICAM-1, VCAM-1
DOI: 10.3233/DMA-2009-0621
Journal: Disease Markers, vol. 26, no. 3, pp. 119-126, 2009
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