Gata-3 and KI-67 expression in correlation with molecular subtypes of breast cancer

Issue title: Second International Conference on Women and Societal Perspective on Quality of Life (WOSQUAL-2020)

Guest editors: Andi Nilawati Usman

Article type: Research Article

Authors: Husni Cangara, M.^a; | Miskad, Upik A.^a | Masadah, Rina^a | Nelwan, Berti J.^a | Wahid, Syarifuddin^a

Affiliations: [a] Department of Anatomical Pathology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

Correspondence: [*] Corresponding author: M. Husni Cangara, Department of Anatomical Pathology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. E-mail: drhusni1977@gmail.com

Abstract: OBJECTIVES:This study aims to evaluate and compare four breast cancer subtypes defined by immunohistochemistry expression of ER, PR, and HER-2 in correlation with Ki-67 and GATA-3 expression. METHODS:Slides from 89 paraffin blocks of invasive breast cancer patients with four molecular subtypes based on HER-2, ER, and PR expression were then stained with Ki-67 and GATA-3 antibodies to evaluate their expression in correlation with molecular subtype and metastases to lymph nodes. RESULTS:This study was a retrospective study of 89 invasive breast cancers. Luminal A; Luminal B; HER2+; and triple-negative types were 35 (39.3%), 10 (11.2%), 27 (30.3%), and 17 (19.1%) samples. Expression of Ki-67 was increased in triple-negative (TN) tumor compared to non-triple-negative (non-TN) tumor subtypes (p < 0.05). This Ki-67 expression was inversely correlated with the positivity of hormone receptor expression related to lymph-node metastases in TN-type tumors. Sixty-two (57%) samples were immunohistochemically positive for GATA-3. GATA-3 positive samples were significantly more likely to be ER and PR-positive, Ki-67 negative, and luminal A tumors. CONCLUSIONS:Subtype triple-negative breast cancer correlates with high expression of Ki-67 that contributes to poor prognosis of this subtype. The higher Ki-67 expression was correlated with the absence of hormone receptor expression compared with the negativity of Her-2 expression, downplay a role in nodal metastases in a triple-negative tumor. GATA-3 positive breast cancer showed luminal differentiation characterized by high ER expression and mainly was classified as luminal A type tumor with a better prognosis.

Keywords: ER, PR, HER-2, KI-67, GATA-3, breast cancer

DOI: 10.3233/BD-219004

Journal: Breast Disease, vol. 40, no. s1, pp. S27-S31, 2021