Investigation on the decolorizing mechanism of pseudomonas sp. R1 on reactive red X-3B
Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 2nd International Conference on Biomedical Engineering and Biotechnology, 11–13 October 2013, Wuhan, China
Affiliations: School of Life Science and Technology, Tongji University, Shanghai, 200092, China | Handan municipal centre for disease control and prevention, Handan, 056000, China | Department of Chemistry, Tongji University, Shanghai, 200092, China
Note: [] Co-first author
Note: [] Co-first author
Note: [] Address for correspondence: Wenwei Tang, Room 437, Department of Chemistry, Tongji University, #1239 Siping Rd, Shanghai, China. Tel.: +86 21 65983366; fax: +86 21 65983366; E-mail: tangww@tongji.edu.cn.
Abstract: A strain of Pseudomonas aeruginosa (Pseudomonas sp. R1), which can efficiently decolorize reactive red X-3B, was isolated from activated sludge in a dye plant, and the decolorizing mechanism was explored in this paper. The result shows that Pseudomonas sp. R1 has very good capability for decolorization of reactive red X-3B and the decolorization rate is increased by 9.1% after optimization of the experimental parameters, which means that 89.6% of the reactive red can be removed. Investigation on decolorization mechanism showed that the decolorizing capability of Pseudomonassp. R1 was significantly affected after plasmids in Pseudomonassp. R1 were eliminated by acridine orange (AO). Meanwhile, E. coli DH5a could gain decolorizing capability after transformed with the plasmids. Plasmid elimination and transformation tests proved that the decolorizing gene in Pseudomonas sp. R1 exists in the plasmid.
