Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuiflora composites

Article type: Research Article

Authors: Limón-Martínez, R.J.^a | Olivas-Armendáriz, I.^a | Sosa-Rodarte, E.^a | Rodríguez-Rodríguez, C.I.^b | Hernández-Paz, J.F.^a | Acosta-Torres, L.S.^c | García-Contreras, R.^c | Santos-Rodríguez, E.^d | Martel-Estrada, S.A.^e;

Affiliations: [a] Instituto de Ingeniería y Tecnología, Universidad Autónoma de Cd. Juárez, Av. Del Charro 450 Norte, Col. Universidad, Cd. Juárez, Chihuahua, México | [b] Universidad Tecnológica de Ciudad Juárez, Av. Universidad Tecnológica 3051, Col. Lote Bravo, Cd. Juárez, Chihuahua, México | [c] Escuela Nacional de Estudios Superiores Unidad León, UNAM, Boulevard UNAM No. 2011, Predio el Saucillo y el Potrero, León Guanajuato, México | [d] ICTP Meso-American Centre for Theoretical Physics (ICTP-MCTP) Universidad Autónoma de Chiapas, Ciudad Universitaria, Carretera Zapata Km. 4, Real del Bosque (Terán), Tuxtla Gutiérrez, Chiapas, México | [e] Instituto de Arquitectura, Diseño y Arte, Universidad Autónoma de Cd. Juárez, Av. Del Charro 450 Norte. Col. Universidad, Cd. Juárez, Chihuahua, México

Correspondence: [*] Corresponding author: S.A. Martel-Estrada, Instituto de Arquitectura, Diseño y Arte, Universidad Autónoma de Cd. Juárez, Av. Del Charro 450 Norte. Col. Universidad, C.P. 32310, Cd. Juárez, Chihuahua, México.  Tel./Fax: +52 656 688 4820; E-mails: mizul@yahoo.com, adriana.martel@uacj.mx

Abstract: BACKGROUND:The development of biomaterial scaffolds and implementation of tissue engineering techniques are necessary. Therefore, Polycaprolactone/Sodium Hyaluronate/Multiwalled Carbon Nanotubes/Extract of Mimosa tenuiflora composites have been produced by a thermally-induced phase separation method. OBJECTIVE:The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites. METHODS:The in vitro bioactivity of the composites was assessed by soaking them in simulated body fluid for 7, 14, 21, and 28 days. The structure and composition of the composites were analyzed using scanning electron microscopy coupled with energy dispersive spectroscopy and Fourier transform infrared spectroscopy. Also, the in vitro biocompatibility of the composites was evaluated by means of alkaline phosphatase activity of the osteoblasts and by measuring the metabolic activity of the cells using MTT assay. RESULTS:The results show a porous and interconnected morphology with enhanced bioactivity. It was observed that the incorporation of Mimosa tenuiflora in the composites promotes increased viability of osteoblasts in the scaffolds. CONCLUSIONS:The results show the efficiency of bioactive and biocompatible composites and their potential as candidates for tissue engineering applications.

Keywords: Bioactive materials, polycaprolactone, sodium Hyaluronate, MWCNT, Mimosa tenuiflora , SBF, MTT assay

DOI: 10.3233/BME-181036

Journal: Bio-Medical Materials and Engineering, vol. 30, no. 1, pp. 97-109, 2019