Affiliations: [a] Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran | [b] Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran | [c] Department of Pharmacology, Iran University of Medical Science, Tehran, Iran | [d] Cellular and Molecular Research Center, Iran University of Medical Science, Tehran, Iran | [e] Tissue Engineering Group (TEG) & Research, National Orthopedic Centre of Excellence in Research & Learning (NOCERAL), Department of Orthopedics, University of Malaya, Kuala Lumpur, Malaysia
Abstract: Background:Recent advances in tissue engineering have led to the development of the concept of bioprinting as an interesting alternative to traditional tissue engineering approaches. Biopaper, a biomimetic hydrogel, is an essential component of the bioprinting process. Objective:The aim of this work was to synthesize a biopaper made of fibrin-gelatin hybrid hydrogel for application in skin bioprinting. Methods:Different composition percentages of the two biopolymer hydrogels, fibrin-gelatin, have been studied for the construction of the biopaper and were examined in terms of water absorption, biodegradability, glucose absorption, mechanical properties and water vapor transmission. Subsequently, tissue fusion study was performed on prepared 3T3 fibroblast cell line pellets embedded into the hydrogel. Results:Based on the obtained results, fibrin-gelatin blend hydrogel with the same proportion of two components provides a natural scaffold for fibroblast-based bioink embedding and culture. Conclusions:The suggested optimized hydrogel was a suitable candidate as a biopaper for skin bioprinting technology.
