Adsorption and Preparation of Human Viruses Using Hydroxyapatite Column

Article type: Research Article

Authors: Tsuru, Sumiaki^; | Shinomiya, Nariyoshi | Katsura, Yoshiya | Uwabe, Yasuhide | Noritake, Masayuki | Rokutanda, Makoto

Affiliations: Department of Microbiology, National Defense Medical College, Saitama, Japan

Note: [] Requests for reprints should be addressed to S. Tsuru, Department of Microbiology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359, Japan.

Abstract: The adsorption and chromatographic properties of hydroxyapatite sorbents for application to different viruses have been investigated. The strong adsorption of viruses was observed on macroporous hydroxyapatite with hydrophilic properties of the sorbent surface. The viruses were purified on this sorbent without loss of biological activity. The column can be used for virus vaccine production.

Keywords: hydroxyapatite column, viral adsorption, viral preparation

DOI: 10.3233/BME-1991-1301

Journal: Bio-Medical Materials and Engineering, vol. 1, no. 3, pp. 143-147, 1991