Cancer Biomarkers - Volume 28, issue 3

Authors: Qin, Ling | Li, Jia-Yu | Huang, Wen-Juan | Zhang, Meng-Lin | Wang, Rui-Tao | Shen, Wenjie

Article Type: Research Article

Abstract: BACKGROUND: Ovarian cancer (OC) is one of the most malignant gynecological cancers. Platelets play a profound role in cancer growth and metastasis. Platelet distribution width (PDW) is an indicator of platelet activation and is altered in malignancies. However, the prognostic value of PDW in OC remains unclear. This present study aimed to investigate the predictive significance of PDW in OC. METHODS: 221 OC patients, between January 2013 and December 2013, were included in this study. The correlations between PDW and clinicopathological features were analyzed. Kaplan-Meier method and Cox regression were used to evaluate the prognostic impact …of PDW. RESULTS: Of the 221 patients, increased PDW levels were observed in 163 (73.6%) patients. Kaplan-Meier analysis revealed that higher PDW levels were associated with poor progression-free survival and overall survival (both p < 0.001). Cox-regression analysis confirmed the independent predictive value of PDW on overall survival (HR = 2.820, 95% CI = 1.776–4.476, p < 0.001). CONCLUSION: Higher PDW levels predict poor prognosis in patients with OC. Elevated PDW may be a novel target for therapy. Show more

Keywords: Ovarian cancer, platelet distribution width, prognosis

DOI: 10.3233/CBM-191190

Citation: Cancer Biomarkers, vol. 28, no. 3, pp. 365-370, 2020

Authors: He, Qing | Zeng, Qiangcheng | Shao, Yibo | Zhou, Haixia | Li, Tianjiao | Song, Fang | Liu, Wei

Article Type: Research Article

Abstract: OBJECTIVES: The purpose of this study was to isolate the secondary metabolites of endophytic fungi from Ginkgo biloba (SMEFGB) and investigate their anti-cervical cancer activity. METHODS: SMEFGB were cultured. The secondary metabolites of endophytic fungi was extracted, purified and identified. The effects of secondary metabolites on proliferation, apoptosis and migration of human cervical cancer HeLa cells were determined. In addition, the effects of SMEFGB on growth of Hela implanted tumor in mice were investigated. RESULTS: In 9 stains of endophytic fungi successfully isolated from the leaves of Ginkgo biloba , the stain …J-1, J-2 and J-3 could produce podophyllotoxin. These 3 stains were identified by molecular biology. The secondary metabolites of stain J-1, J-2 and J-3 markedly inhibited the proliferation of HeLa cells, promoted their apoptosis and blocked their migration. In addition, the secondary metabolites of stain J-1, J-2 and J-3 significantly attenuated the growth of HeLa implanted tumor in mice. CONCLUSIONS: Our results indicated that SMEFGB had obvious anti-cervical cancer activity in vitro and in vivo . Show more

Keywords: Secondary metabolites, endophytic fungi, Ginkgo biloba, cervical cancer, anti-cervical cancer activity

DOI: 10.3233/CBM-190462

Citation: Cancer Biomarkers, vol. 28, no. 3, pp. 371-379, 2020

Authors: Zhang, Pei | Hou, Qingxia | Yue, Qingfen

Article Type: Research Article

Abstract: MicroRNAs (MiRNAs) have been clarified as crucial regulators of the pathological processes in various carcinomas in the past years. Interestingly, existing evidence has manifested that microRNA-204-5p (miR-204-5p) is engaged in the initiation and progression of multiple carcinomas. However, the potential of miR-204-5p in cervical cancer remains to be disentombed. This study focused on unraveling the detailed role of miR-204-5p in cervical cancer. MiR-204-5p exhibited a low level in cervical cancer cells. The functional assays demonstrated that miR-204-5p upregulation exerted suppressive impact on the functions of cervical cancer cells, including proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) process. Moreover, transcription factor …AP-2 alpha (TFAP2A) was screened to be the most affected target gene by miR-204-5p, and TFAP2A was discovered to transcriptionally repress miR-204-5p in cervical cancer. The mutual regulation between TFAP2A and miR-204-5p was testified through molecular mechanism assays. Final rescued-function assays demonstrated that overexpression of TFAP2A could recover the suppressed cellular process caused by miR-204-5p upregulation. In conclusion, miR-204-5p/TFAP2A feedback loop promoted the proliferative and motorial capacities of cervical cancer cells. This finding suggested a novel modulatory loop of miR-204-5p/TFAP2A in cervical cancer, offering promising biomarkers for cervical cancer therapy. Show more

Keywords: Cervical cancer, miR-204-5p, TFAP2A, feedback loop

DOI: 10.3233/CBM-191064

Citation: Cancer Biomarkers, vol. 28, no. 3, pp. 381-390, 2020

Authors: Li, Bo | Shen, Kexin | Zhang, Jiayu | Jiang, Yang | Yang, Ting | Sun, Xiaoxu | Ma, Xiaoming | Zhu, Jinzhou

Article Type: Research Article

Abstract: BACKGROUND: Recent evidence support that netrin-1 involves in colorectal carcinogenesis. OBJECTIVE: This study was to evaluate the performance of serum netrin-1 for detection of colorectal cancer (CRC) in both clinical/screening sets. METHODS: A total of 115 consecutive patients with CRC and matched healthy controls were included in Clinical Set. Fifty subjects with CRC, 50 subjects with advanced adenoma (AA), and 150 matched control participants free of neoplasia were included in Screening Set. RESULTS: In Clinical set, subjects with CRC presented higher levels of serum netrin-1 (513.9 ± …22.6 pg/mL) than controls (347.8 ± 20.3 pg/mL, p < 0.0001). Similar in Screening set, serum netrin-1 was higher in CRC (644.5 ± 37.0 pg/mL, both p < 0.0001), compared with controls (407.7 ± 14.8 pg/mL) and AA (416.5 ± 18.5 pg/mL). However, there was no difference between controls and AA (p = 0.752). Compared with the low netrin-1 group, the high group presented increased risk of CRC (Clinical set: OR = 4.300, p < 0.001; Screening set: OR = 7.731, p < 0.001). ROC curve of netrin-1 was developed to detect CRC (Clinical set: AUC 0.703; Screening set: AUC 0.759). CONCLUSIONS: It suggests netrin-1 as a potential biomarker for CRC detection. Show more

Keywords: Biomarker, netrin-1, colorectal cancer, screening

DOI: 10.3233/CBM-190340

Citation: Cancer Biomarkers, vol. 28, no. 3, pp. 391-396, 2020

Authors: Nagare, Rohit Pravin | Sneha, Smarakan | Sidhanth, Chirukandath | Roopa, S. | Murhekar, Kanchan | Shirley, Sundersingh | Swaminathan, Rajaraman | Sridevi, Velusamy | Ganesan, Trivadi Sundaram

Article Type: Research Article

Abstract: BACKGROUND: There has been variability between laboratories in the identification of cancer stem cells (CSCs) markers for epithelial ovarian cancer (EOC). We have evaluated three new surface markers for EOC to identify CSCs precisely. METHODS: Three new putative CSCs specific surface markers CD9 , CD24 and EPHA1 identified by a bioinformatics approach were evaluated in normal ovary, fallopian tube and ovarian tumours. RESULTS: The expression of CD9 alone was observed in normal ovarian surface epithelium and fallopian tube whereas CD24 and EPHA1 were not expressed (n = …5). CD24 was expressed in all tumours (N = 101) while CD9 and EPHA1 were expressed in 89 and 71 tumours, respectively. The statistical analysis showed significant correlation of the stage of the disease (p < 0.0001), type of surgery (p < 0.0001) and residual disease (p < 0.0001) with overall survival. Although expression of CD9 , CD24 and EPHA1 was observed in the majority of tumours there was no significant correlation with outcome. In patients who underwent primary surgery, increased expression of CD24 significantly correlated with poor survival. The expression of CD24 was significantly reduced (p < 0.002) upon analysis of paired sections from patients prior to surgery and at interval debulking surgery (n = 16). CONCLUSION: These findings suggest that overexpression of these new markers may be useful in identifying and targeting ovarian CSCs and CD24 may be a putative CSCs marker in ovarian cancer. Show more

Keywords: Ovarian cancer stem cells, serous ovarian cancer, CD24, CD9, EPHA1, primary surgery, interval debulking surgery, clinical outcome

DOI: 10.3233/CBM-201463

Citation: Cancer Biomarkers, vol. 28, no. 3, pp. 397-408, 2020

Article Type: Correction

DOI: 10.3233/CBM-169715

Citation: Cancer Biomarkers, vol. 28, no. 3, pp. 409-, 2020